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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacteroides vulgatus Arginine--tRNA ligase (argS), partial CSB-YP406370BOP
CSB-EP406370BOP
CSB-BP406370BOP
CSB-MP406370BOP
CSB-EP406370BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP406371BOP
CSB-EP406371BOP
CSB-BP406371BOP
CSB-MP406371BOP
CSB-EP406371BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Ribonuclease HII (rnhB) CSB-YP406372BOP
CSB-EP406372BOP
CSB-BP406372BOP
CSB-MP406372BOP
CSB-EP406372BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP406373BOP
CSB-EP406373BOP
CSB-BP406373BOP
CSB-MP406373BOP
CSB-EP406373BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus 30S ribosomal protein S2 (rpsB) CSB-YP406374BOP
CSB-EP406374BOP
CSB-BP406374BOP
CSB-MP406374BOP
CSB-EP406374BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus NADH-quinone oxidoreductase subunit C/D (nuoC), partial CSB-YP406375BOP
CSB-EP406375BOP
CSB-BP406375BOP
CSB-MP406375BOP
CSB-EP406375BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP406376BOP
CSB-EP406376BOP
CSB-BP406376BOP
CSB-MP406376BOP
CSB-EP406376BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus UPF0102 protein BVU_1879 (BVU_1879) CSB-YP406377BOP
CSB-EP406377BOP
CSB-BP406377BOP
CSB-MP406377BOP
CSB-EP406377BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Cytidylate kinase (cmk) CSB-YP406378BOP
CSB-EP406378BOP
CSB-BP406378BOP
CSB-MP406378BOP
CSB-EP406378BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Glycosyl hydrolase family 109 protein 5 (BVU_2041) CSB-YP406379BOP
CSB-EP406379BOP
CSB-BP406379BOP
CSB-MP406379BOP
CSB-EP406379BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Maf-like protein BVU_2498 (BVU_2498) CSB-YP406380BOP
CSB-EP406380BOP
CSB-BP406380BOP
CSB-MP406380BOP
CSB-EP406380BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Ribonuclease Z (rnz) CSB-YP406381BOP
CSB-EP406381BOP
CSB-BP406381BOP
CSB-MP406381BOP
CSB-EP406381BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP406382BOP
CSB-EP406382BOP
CSB-BP406382BOP
CSB-MP406382BOP
CSB-EP406382BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Aspartate 1-decarboxylase (panD) CSB-YP406383BOP
CSB-EP406383BOP
CSB-BP406383BOP
CSB-MP406383BOP
CSB-EP406383BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus Formate--tetrahydrofolate ligase (fhs), partial CSB-YP406384BOP
CSB-EP406384BOP
CSB-BP406384BOP
CSB-MP406384BOP
CSB-EP406384BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP406385BOP
CSB-EP406385BOP
CSB-BP406385BOP
CSB-MP406385BOP
CSB-EP406385BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus 50S ribosomal protein L33 (rpmG) CSB-YP406386BOP
CSB-EP406386BOP
CSB-BP406386BOP
CSB-MP406386BOP
CSB-EP406386BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP406387BOP
CSB-EP406387BOP
CSB-BP406387BOP
CSB-MP406387BOP
CSB-EP406387BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus tRNA dimethylallyltransferase 2 (miaA2) CSB-YP406388BOP
CSB-EP406388BOP
CSB-BP406388BOP
CSB-MP406388BOP
CSB-EP406388BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides vulgatus 50S ribosomal protein L25 (rplY) CSB-YP406389BOP
CSB-EP406389BOP
CSB-BP406389BOP
CSB-MP406389BOP
CSB-EP406389BOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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