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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia mallei 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP380620BPR
CSB-EP380620BPR
CSB-BP380620BPR
CSB-MP380620BPR
CSB-EP380620BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Tryptophan synthase alpha chain (trpA) CSB-YP380621BPR
CSB-EP380621BPR
CSB-BP380621BPR
CSB-MP380621BPR
CSB-EP380621BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei HPr kinase/phosphorylase (hprK) CSB-YP380622BPR
CSB-EP380622BPR
CSB-BP380622BPR
CSB-MP380622BPR
CSB-EP380622BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP380623BPR
CSB-EP380623BPR
CSB-BP380623BPR
CSB-MP380623BPR
CSB-EP380623BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP380624BPR
CSB-EP380624BPR
CSB-BP380624BPR
CSB-MP380624BPR
CSB-EP380624BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Octanoyltransferase (lipB) CSB-YP380625BPR
CSB-EP380625BPR
CSB-BP380625BPR
CSB-MP380625BPR
CSB-EP380625BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei D-tyrosyl-tRNA (Tyr) deacylase CSB-YP380626BPR
CSB-EP380626BPR
CSB-BP380626BPR
CSB-MP380626BPR
CSB-EP380626BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP380627BPR
CSB-EP380627BPR
CSB-BP380627BPR
CSB-MP380627BPR
CSB-EP380627BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Catalase-peroxidase (katG), partial CSB-YP380628BPR
CSB-EP380628BPR
CSB-BP380628BPR
CSB-MP380628BPR
CSB-EP380628BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Cyanate hydratase (cynS) CSB-YP380629BPR
CSB-EP380629BPR
CSB-BP380629BPR
CSB-MP380629BPR
CSB-EP380629BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei GTPase obg (obg) CSB-YP380630BPR
CSB-EP380630BPR
CSB-BP380630BPR
CSB-MP380630BPR
CSB-EP380630BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Protein translocase subunit SecA (secA), partial CSB-YP380631BPR
CSB-EP380631BPR
CSB-BP380631BPR
CSB-MP380631BPR
CSB-EP380631BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD), partial CSB-YP380632BPR
CSB-EP380632BPR
CSB-BP380632BPR
CSB-MP380632BPR
CSB-EP380632BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP380633BPR
CSB-EP380633BPR
CSB-BP380633BPR
CSB-MP380633BPR
CSB-EP380633BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Chaperone protein DnaJ (dnaJ) CSB-YP380634BPR
CSB-EP380634BPR
CSB-BP380634BPR
CSB-MP380634BPR
CSB-EP380634BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP380635BPR
CSB-EP380635BPR
CSB-BP380635BPR
CSB-MP380635BPR
CSB-EP380635BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Urease subunit alpha (ureC), partial CSB-YP380636BPR
CSB-EP380636BPR
CSB-BP380636BPR
CSB-MP380636BPR
CSB-EP380636BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Protein CrcB homolog (crcB), partial CSB-YP380637BPR1
CSB-EP380637BPR1
CSB-BP380637BPR1
CSB-MP380637BPR1
CSB-EP380637BPR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP380638BPR
CSB-EP380638BPR
CSB-BP380638BPR
CSB-MP380638BPR
CSB-EP380638BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Homogentisate 1,2-dioxygenase (hmgA) CSB-YP380639BPR
CSB-EP380639BPR
CSB-BP380639BPR
CSB-MP380639BPR
CSB-EP380639BPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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