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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Polaromonas naphthalenivorans Histidinol-phosphate aminotransferase (hisC) CSB-YP379800PYT
CSB-EP379800PYT
CSB-BP379800PYT
CSB-MP379800PYT
CSB-EP379800PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP379801PYT
CSB-EP379801PYT
CSB-BP379801PYT
CSB-MP379801PYT
CSB-EP379801PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Protein translocase subunit SecA (secA), partial CSB-YP379802PYT
CSB-EP379802PYT
CSB-BP379802PYT
CSB-MP379802PYT
CSB-EP379802PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans GTPase obg (obg) CSB-YP379803PYT
CSB-EP379803PYT
CSB-BP379803PYT
CSB-MP379803PYT
CSB-EP379803PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP379804PYT
CSB-EP379804PYT
CSB-BP379804PYT
CSB-MP379804PYT
CSB-EP379804PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP379805PYT
CSB-EP379805PYT
CSB-BP379805PYT
CSB-MP379805PYT
CSB-EP379805PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Protein-export protein secB 1 (secB1) CSB-YP379806PYT
CSB-EP379806PYT
CSB-BP379806PYT
CSB-MP379806PYT
CSB-EP379806PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Aspartate 1-decarboxylase (panD) CSB-YP379807PYT
CSB-EP379807PYT
CSB-BP379807PYT
CSB-MP379807PYT
CSB-EP379807PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Urease accessory protein UreE (ureE) CSB-YP379808PYT
CSB-EP379808PYT
CSB-BP379808PYT
CSB-MP379808PYT
CSB-EP379808PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Urease subunit gamma (ureA) CSB-YP379809PYT
CSB-EP379809PYT
CSB-BP379809PYT
CSB-MP379809PYT
CSB-EP379809PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans 30S ribosomal protein S21 (rpsU) CSB-YP379810PYT
CSB-EP379810PYT
CSB-BP379810PYT
CSB-MP379810PYT
CSB-EP379810PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP379811PYT
CSB-EP379811PYT
CSB-BP379811PYT
CSB-MP379811PYT
CSB-EP379811PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP379812PYT
CSB-EP379812PYT
CSB-BP379812PYT
CSB-MP379812PYT
CSB-EP379812PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Ribosome maturation factor RimM (rimM) CSB-YP379813PYT
CSB-EP379813PYT
CSB-BP379813PYT
CSB-MP379813PYT
CSB-EP379813PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Chaperone protein DnaK (dnaK), partial CSB-YP379814PYT
CSB-EP379814PYT
CSB-BP379814PYT
CSB-MP379814PYT
CSB-EP379814PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Disulfide bond formation protein B (dsbB), partial CSB-YP379815PYT1
CSB-EP379815PYT1
CSB-BP379815PYT1
CSB-MP379815PYT1
CSB-EP379815PYT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Recombination protein RecR (recR) CSB-YP379816PYT
CSB-EP379816PYT
CSB-BP379816PYT
CSB-MP379816PYT
CSB-EP379816PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Ketol-acid reductoisomerase (ilvC) CSB-YP379817PYT
CSB-EP379817PYT
CSB-BP379817PYT
CSB-MP379817PYT
CSB-EP379817PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Uracil phosphoribosyltransferase (upp) CSB-YP379818PYT
CSB-EP379818PYT
CSB-BP379818PYT
CSB-MP379818PYT
CSB-EP379818PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polaromonas naphthalenivorans Uridylate kinase (pyrH) CSB-YP379819PYT
CSB-EP379819PYT
CSB-BP379819PYT
CSB-MP379819PYT
CSB-EP379819PYT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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