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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Acidovorax sp. NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP379480AUC
CSB-EP379480AUC
CSB-BP379480AUC
CSB-MP379480AUC
CSB-EP379480AUC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP379481AUC
CSB-EP379481AUC
CSB-BP379481AUC
CSB-MP379481AUC
CSB-EP379481AUC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Deoxycytidine triphosphate deaminase (dcd) CSB-YP379482AUC
CSB-EP379482AUC
CSB-BP379482AUC
CSB-MP379482AUC
CSB-EP379482AUC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Undecaprenyl-diphosphatase (uppP), partial CSB-YP379483AUC1
CSB-EP379483AUC1
CSB-BP379483AUC1
CSB-MP379483AUC1
CSB-EP379483AUC1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. GTPase Der (der) CSB-YP379484AUC
CSB-EP379484AUC
CSB-BP379484AUC
CSB-MP379484AUC
CSB-EP379484AUC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Trigger factor (tig) CSB-YP379485AUC
CSB-EP379485AUC
CSB-BP379485AUC
CSB-MP379485AUC
CSB-EP379485AUC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. UPF0060 membrane protein Ajs_1326 (Ajs_1326), partial CSB-YP379486AUC1
CSB-EP379486AUC1
CSB-BP379486AUC1
CSB-MP379486AUC1
CSB-EP379486AUC1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. 3-dehydroquinate dehydratase (aroQ) CSB-YP379487AUC
CSB-EP379487AUC
CSB-BP379487AUC
CSB-MP379487AUC
CSB-EP379487AUC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Probable intracellular septation protein A (Ajs_1675), partial CSB-YP379488AUC1
CSB-EP379488AUC1
CSB-BP379488AUC1
CSB-MP379488AUC1
CSB-EP379488AUC1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Probable molybdopterin-guanine dinucleotide biosynthesis protein A (mobA) CSB-YP379489AUC
CSB-EP379489AUC
CSB-BP379489AUC
CSB-MP379489AUC
CSB-EP379489AUC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Ferredoxin--NADP reductase (Ajs_1789) CSB-YP379490AUC
CSB-EP379490AUC
CSB-BP379490AUC
CSB-MP379490AUC
CSB-EP379490AUC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP379491AUC
CSB-EP379491AUC
CSB-BP379491AUC
CSB-MP379491AUC
CSB-EP379491AUC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. tRNA (guanine-N (7)-)-methyltransferase CSB-YP379492AUC
CSB-EP379492AUC
CSB-BP379492AUC
CSB-MP379492AUC
CSB-EP379492AUC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP379493AUC
CSB-EP379493AUC
CSB-BP379493AUC
CSB-MP379493AUC
CSB-EP379493AUC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Dihydroorotate dehydrogenase (quinone) CSB-YP379494AUC
CSB-EP379494AUC
CSB-BP379494AUC
CSB-MP379494AUC
CSB-EP379494AUC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Chaperone protein hscA homolog (hscA), partial CSB-YP379495AUC
CSB-EP379495AUC
CSB-BP379495AUC
CSB-MP379495AUC
CSB-EP379495AUC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP379496AUC
CSB-EP379496AUC
CSB-BP379496AUC
CSB-MP379496AUC
CSB-EP379496AUC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. LexA repressor (lexA) CSB-YP379497AUC
CSB-EP379497AUC
CSB-BP379497AUC
CSB-MP379497AUC
CSB-EP379497AUC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP379498AUC
CSB-EP379498AUC
CSB-BP379498AUC
CSB-MP379498AUC
CSB-EP379498AUC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidovorax sp. Threonine--tRNA ligase (thrS), partial CSB-YP379499AUC
CSB-EP379499AUC
CSB-BP379499AUC
CSB-MP379499AUC
CSB-EP379499AUC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務(wù)，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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