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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella sp. Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP376179STT
CSB-EP376179STT
CSB-BP376179STT
CSB-MP376179STT
CSB-EP376179STT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. 30S ribosomal protein S15 (rpsO) CSB-YP376180STT
CSB-EP376180STT
CSB-BP376180STT
CSB-MP376180STT
CSB-EP376180STT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Transcriptional repressor NrdR (nrdR) CSB-YP376181STT
CSB-EP376181STT
CSB-BP376181STT
CSB-MP376181STT
CSB-EP376181STT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Cell division protein FtsB (ftsB), partial CSB-YP376182STT
CSB-EP376182STT
CSB-BP376182STT
CSB-MP376182STT
CSB-EP376182STT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP376183STT
CSB-EP376183STT
CSB-BP376183STT
CSB-MP376183STT
CSB-EP376183STT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Carbon storage regulator homolog (csrA) CSB-YP376184STT
CSB-EP376184STT
CSB-BP376184STT
CSB-MP376184STT
CSB-EP376184STT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP376185STT
CSB-EP376185STT
CSB-BP376185STT
CSB-MP376185STT
CSB-EP376185STT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Serine/threonine transporter sstT (sstT), partial CSB-YP376186STT
CSB-EP376186STT
CSB-BP376186STT
CSB-MP376186STT
CSB-EP376186STT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. UPF0115 protein Sputw3181_1555 (Sputw3181_1555) CSB-YP376187STT
CSB-EP376187STT
CSB-BP376187STT
CSB-MP376187STT
CSB-EP376187STT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Tryptophan synthase beta chain (trpB) CSB-YP376188STT
CSB-EP376188STT
CSB-BP376188STT
CSB-MP376188STT
CSB-EP376188STT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. DNA ligase (ligA), partial CSB-YP376189STT
CSB-EP376189STT
CSB-BP376189STT
CSB-MP376189STT
CSB-EP376189STT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Biotin synthase (bioB) CSB-YP376190STT
CSB-EP376190STT
CSB-BP376190STT
CSB-MP376190STT
CSB-EP376190STT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Protease HtpX (htpX), partial CSB-YP376191STT1
CSB-EP376191STT1
CSB-BP376191STT1
CSB-MP376191STT1
CSB-EP376191STT1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Recombination protein RecR (recR) CSB-YP376192STT
CSB-EP376192STT
CSB-BP376192STT
CSB-MP376192STT
CSB-EP376192STT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Adenylate kinase (adk) CSB-YP376193STT
CSB-EP376193STT
CSB-BP376193STT
CSB-MP376193STT
CSB-EP376193STT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Nucleoid-associated protein Sputw3181_1720 (Sputw3181_1720) CSB-YP376194STT
CSB-EP376194STT
CSB-BP376194STT
CSB-MP376194STT
CSB-EP376194STT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP376195STT
CSB-EP376195STT
CSB-BP376195STT
CSB-MP376195STT
CSB-EP376195STT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. UPF0176 protein Sputw3181_1881 (Sputw3181_1881) CSB-YP376196STT
CSB-EP376196STT
CSB-BP376196STT
CSB-MP376196STT
CSB-EP376196STT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Glucans biosynthesis glucosyltransferase H (opgH), partial CSB-YP376197STT1
CSB-EP376197STT1
CSB-BP376197STT1
CSB-MP376197STT1
CSB-EP376197STT1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. L-arabinose isomerase (araA) CSB-YP376198STT
CSB-EP376198STT
CSB-BP376198STT
CSB-MP376198STT
CSB-EP376198STT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務(wù)，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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