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重組蛋白

產品名稱 貨號 規格
Recombinant Bacillus thuringiensis Heptaprenylglyceryl phosphate synthase (pcrB) CSB-YP371165BOM
CSB-EP371165BOM
CSB-BP371165BOM
CSB-MP371165BOM
CSB-EP371165BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP371166BOM
CSB-EP371166BOM
CSB-BP371166BOM
CSB-MP371166BOM
CSB-EP371166BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP371167BOM
CSB-EP371167BOM
CSB-BP371167BOM
CSB-MP371167BOM
CSB-EP371167BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis DNA topoisomerase 3 (topB), partial CSB-YP371168BOM
CSB-EP371168BOM
CSB-BP371168BOM
CSB-MP371168BOM
CSB-EP371168BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Proline--tRNA ligase 2 (proS2) CSB-YP371169BOM
CSB-EP371169BOM
CSB-BP371169BOM
CSB-MP371169BOM
CSB-EP371169BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Regulatory protein recX (recX) CSB-YP371170BOM
CSB-EP371170BOM
CSB-BP371170BOM
CSB-MP371170BOM
CSB-EP371170BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Glutamate-1-semialdehyde 2,1-aminomutase 1 (hemL1) CSB-YP371171BOM
CSB-EP371171BOM
CSB-BP371171BOM
CSB-MP371171BOM
CSB-EP371171BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Undecaprenyl-diphosphatase 2 (uppP2), partial CSB-YP371172BOM1
CSB-EP371172BOM1
CSB-BP371172BOM1
CSB-MP371172BOM1
CSB-EP371172BOM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Potassium-transporting ATPase A chain (kdpA), partial CSB-YP371173BOM
CSB-EP371173BOM
CSB-BP371173BOM
CSB-MP371173BOM
CSB-EP371173BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis UPF0754 membrane protein BALH_0780 (BALH_0780), partial CSB-YP371174BOM1
CSB-EP371174BOM1
CSB-BP371174BOM1
CSB-MP371174BOM1
CSB-EP371174BOM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Protein translocase subunit SecA 2 (secA2), partial CSB-YP371175BOM
CSB-EP371175BOM
CSB-BP371175BOM
CSB-MP371175BOM
CSB-EP371175BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 3'-5' exoribonuclease yhaM (yhaM) CSB-YP371176BOM
CSB-EP371176BOM
CSB-BP371176BOM
CSB-MP371176BOM
CSB-EP371176BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP371177BOM
CSB-EP371177BOM
CSB-BP371177BOM
CSB-MP371177BOM
CSB-EP371177BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis UPF0738 protein BALH_1059 (BALH_1059) CSB-YP371178BOM
CSB-EP371178BOM
CSB-BP371178BOM
CSB-MP371178BOM
CSB-EP371178BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Anthranilate phosphoribosyltransferase (trpD) CSB-YP371179BOM
CSB-EP371179BOM
CSB-BP371179BOM
CSB-MP371179BOM
CSB-EP371179BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Indole-3-glycerol phosphate synthase (trpC) CSB-YP371180BOM
CSB-EP371180BOM
CSB-BP371180BOM
CSB-MP371180BOM
CSB-EP371180BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Spermidine/putrescine import ATP-binding protein PotA (potA) CSB-YP371181BOM
CSB-EP371181BOM
CSB-BP371181BOM
CSB-MP371181BOM
CSB-EP371181BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Lactate utilization protein A (lutA) CSB-YP371182BOM
CSB-EP371182BOM
CSB-BP371182BOM
CSB-MP371182BOM
CSB-EP371182BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 2-aminoethylphosphonate--pyruvate transaminase (phnW) CSB-YP371183BOM
CSB-EP371183BOM
CSB-BP371183BOM
CSB-MP371183BOM
CSB-EP371183BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis D-alanine--poly (phosphoribitol) ligase subunit 1, partial CSB-YP371184BOM
CSB-EP371184BOM
CSB-BP371184BOM
CSB-MP371184BOM
CSB-EP371184BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業產品。重組蛋白的技術資源在重組蛋白的開發過程中,除了表達系統之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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