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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bovine Neuropeptide-like protein C4orf48 homolog CSB-YP368835BO
CSB-EP368835BO
CSB-BP368835BO
CSB-MP368835BO
CSB-EP368835BO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis UPF0515 protein C19orf66 homolog CSB-YP368836XBF
CSB-EP368836XBF
CSB-BP368836XBF
CSB-MP368836XBF
CSB-EP368836XBF-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Membrane protein FAM174 (fam174), partial CSB-YP368837XBF
CSB-EP368837XBF
CSB-BP368837XBF
CSB-MP368837XBF
CSB-EP368837XBF-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase-like protein (ispd) CSB-YP368838DIL
CSB-EP368838DIL
CSB-BP368838DIL
CSB-MP368838DIL
CSB-EP368838DIL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Centrosomal protein of 41 kDa B (cep41-b) CSB-YP368839XBE
CSB-EP368839XBE
CSB-BP368839XBE
CSB-MP368839XBE
CSB-EP368839XBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Na (+)/H (+) antiporter nhaA, partial CSB-YP368840AVA
CSB-EP368840AVA
CSB-BP368840AVA
CSB-MP368840AVA
CSB-EP368840AVA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Urease subunit gamma (ureA) CSB-YP368841AVA
CSB-EP368841AVA
CSB-BP368841AVA
CSB-MP368841AVA
CSB-EP368841AVA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Urease subunit alpha (ureC), partial CSB-YP368842AVA
CSB-EP368842AVA
CSB-BP368842AVA
CSB-MP368842AVA
CSB-EP368842AVA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Adenylosuccinate synthetase (purA) CSB-YP368843AVA
CSB-EP368843AVA
CSB-BP368843AVA
CSB-MP368843AVA
CSB-EP368843AVA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP368844AVA
CSB-EP368844AVA
CSB-BP368844AVA
CSB-MP368844AVA
CSB-EP368844AVA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Inositol 2-dehydrogenase (iolG) CSB-YP368845AVA
CSB-EP368845AVA
CSB-BP368845AVA
CSB-MP368845AVA
CSB-EP368845AVA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Bifunctional protein FolD (folD) CSB-YP368846AVA
CSB-EP368846AVA
CSB-BP368846AVA
CSB-MP368846AVA
CSB-EP368846AVA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Peptidyl-tRNA hydrolase (pth) CSB-YP368847AVA
CSB-EP368847AVA
CSB-BP368847AVA
CSB-MP368847AVA
CSB-EP368847AVA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Imidazolonepropionase (hutI) CSB-YP368848AVA
CSB-EP368848AVA
CSB-BP368848AVA
CSB-MP368848AVA
CSB-EP368848AVA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Ribosome maturation factor RimP (rimP) CSB-YP368849AVA
CSB-EP368849AVA
CSB-BP368849AVA
CSB-MP368849AVA
CSB-EP368849AVA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. tRNA pseudouridine synthase B (truB) CSB-YP368850AVA
CSB-EP368850AVA
CSB-BP368850AVA
CSB-MP368850AVA
CSB-EP368850AVA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Protein RecA (recA) CSB-YP368851AVA
CSB-EP368851AVA
CSB-BP368851AVA
CSB-MP368851AVA
CSB-EP368851AVA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP368852AVA
CSB-EP368852AVA
CSB-BP368852AVA
CSB-MP368852AVA
CSB-EP368852AVA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. 50S ribosomal protein L20 (rplT) CSB-YP368853AVA
CSB-EP368853AVA
CSB-BP368853AVA
CSB-MP368853AVA
CSB-EP368853AVA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Cell division protein SepF (sepF) CSB-YP368854AVA
CSB-EP368854AVA
CSB-BP368854AVA
CSB-MP368854AVA
CSB-EP368854AVA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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