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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vitis vinifera Acidic endochitinase (CHIT3) CSB-YP343169VFQ
CSB-EP343169VFQ
CSB-BP343169VFQ
CSB-MP343169VFQ
CSB-EP343169VFQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Magnesium-chelatase subunit ChlI (chlI) CSB-YP343170SSQ
CSB-EP343170SSQ
CSB-BP343170SSQ
CSB-MP343170SSQ
CSB-EP343170SSQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus phage HP1 Uncharacterized 8.9 kDa protein in int-C1 intergenic region CSB-YP343171HAN
CSB-EP343171HAN
CSB-BP343171HAN
CSB-MP343171HAN
CSB-EP343171HAN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus phage HP1 Uncharacterized 18.2 kDa protein in rep-hol intergenic region CSB-YP343172HAN
CSB-EP343172HAN
CSB-BP343172HAN
CSB-MP343172HAN
CSB-EP343172HAN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus phage HP1 Probable head completion/stabilization protein CSB-YP343173HAN
CSB-EP343173HAN
CSB-BP343173HAN
CSB-MP343173HAN
CSB-EP343173HAN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cervus elaphus Tumor necrosis factor (TNF), partial CSB-YP343176DRN
CSB-EP343176DRN
CSB-BP343176DRN
CSB-MP343176DRN
CSB-EP343176DRN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon kondoi 50S ribosomal protein L6 (rplF) CSB-YP343177BMY
CSB-EP343177BMY
CSB-BP343177BMY
CSB-MP343177BMY
CSB-EP343177BMY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus elongatus Uncharacterized protein Synpcc7942_1424 (Synpcc7942_1424) CSB-YP343178FPY
CSB-EP343178FPY
CSB-BP343178FPY
CSB-MP343178FPY
CSB-EP343178FPY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein usf (usf) CSB-YP343179DNW
CSB-EP343179DNW
CSB-BP343179DNW
CSB-MP343179DNW
CSB-EP343179DNW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative histidine--tRNA ligase (hisS) CSB-YP343180FPJ
CSB-EP343180FPJ
CSB-BP343180FPJ
CSB-MP343180FPJ
CSB-EP343180FPJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Transcriptional repressor NrdR (nrdR) CSB-YP343181FPZ
CSB-EP343181FPZ
CSB-BP343181FPZ
CSB-MP343181FPZ
CSB-EP343181FPZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fusarium oxysporum Endoglucanase type C CSB-YP343182FFO
CSB-EP343182FFO
CSB-BP343182FFO
CSB-MP343182FFO
CSB-EP343182FFO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Threonine--tRNA ligase (thrS), partial CSB-YP343183BXE
CSB-EP343183BXE
CSB-BP343183BXE
CSB-MP343183BXE
CSB-EP343183BXE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays 60S acidic ribosomal protein P2A (RPP2A) CSB-YP343184ZAX
CSB-EP343184ZAX
CSB-BP343184ZAX
CSB-MP343184ZAX
CSB-EP343184ZAX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Cytochrome b-c1 complex subunit 9 CSB-YP343185FIG
CSB-EP343185FIG
CSB-BP343185FIG
CSB-MP343185FIG
CSB-EP343185FIG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Sedoheptulose-1,7-bisphosphatase, chloroplastic CSB-YP343186TQN
CSB-EP343186TQN
CSB-BP343186TQN
CSB-MP343186TQN
CSB-EP343186TQN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas moewusii Photosystem II reaction center protein L (psbL), partial CSB-YP343187CIX
CSB-EP343187CIX
CSB-BP343187CIX
CSB-MP343187CIX
CSB-EP343187CIX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein ycf22 (ycf22) CSB-YP343188ALT
CSB-EP343188ALT
CSB-BP343188ALT
CSB-MP343188ALT
CSB-EP343188ALT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis HTH-type transcriptional regulator iolR (iolR) CSB-YP343189BRJ
CSB-EP343189BRJ
CSB-BP343189BRJ
CSB-MP343189BRJ
CSB-EP343189BRJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis RNA-binding protein YbxF (rulS),Yeast CSB-YP343190BRJ
CSB-BP343190BRJ
CSB-MP343190BRJ
CSB-EP343190BRJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務(wù)，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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