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重組蛋白

產品名稱 貨號 規格
Recombinant Saccharomyces cerevisiae Protein HPH2 (FRT2), partial CSB-YP331068SVG1
CSB-EP331068SVG1
CSB-BP331068SVG1
CSB-MP331068SVG1
CSB-EP331068SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Flagellar protein fliS (fliS) CSB-YP331069BRJ
CSB-EP331069BRJ
CSB-BP331069BRJ
CSB-MP331069BRJ
CSB-EP331069BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Translocation protein SEC72 (SEC72) CSB-YP331070SVG
CSB-EP331070SVG
CSB-BP331070SVG
CSB-MP331070SVG
CSB-EP331070SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative transition state regulator Abh (abh) CSB-YP331071BRJ
CSB-EP331071BRJ
CSB-BP331071BRJ
CSB-MP331071BRJ
CSB-EP331071BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uracil permease (pyrP), partial CSB-YP331072BRJ
CSB-EP331072BRJ
CSB-BP331072BRJ
CSB-MP331072BRJ
CSB-EP331072BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Polyhomeotic-proximal chromatin protein (ph-p), partial CSB-YP331073DLU
CSB-EP331073DLU
CSB-BP331073DLU
CSB-MP331073DLU
CSB-EP331073DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP331074BRJ
CSB-EP331074BRJ
CSB-BP331074BRJ
CSB-MP331074BRJ
CSB-EP331074BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis GTP-sensing transcriptional pleiotropic repressor CodY (codY) CSB-YP331075BRJ
CSB-EP331075BRJ
CSB-BP331075BRJ
CSB-MP331075BRJ
CSB-EP331075BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable endonuclease III (nth) CSB-YP331076BRJ
CSB-EP331076BRJ
CSB-BP331076BRJ
CSB-MP331076BRJ
CSB-EP331076BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Penicillin-binding protein 1A/1B (ponA), partial CSB-YP331077BRJ
CSB-BP331077BRJ
CSB-MP331077BRJ
CSB-EP331077BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Phage-like element PBSX protein xepA (xepA) CSB-YP331078BRJ
CSB-EP331078BRJ
CSB-BP331078BRJ
CSB-MP331078BRJ
CSB-EP331078BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Intracellular proteinase inhibitor (ipi) CSB-YP331079BRJ
CSB-EP331079BRJ
CSB-BP331079BRJ
CSB-MP331079BRJ
CSB-EP331079BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yvyG (yvyG) CSB-YP331080BRJ
CSB-EP331080BRJ
CSB-BP331080BRJ
CSB-MP331080BRJ
CSB-EP331080BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glutamate synthase [NADPH] large chain (gltA), partial CSB-YP331081BRJ
CSB-EP331081BRJ
CSB-BP331081BRJ
CSB-MP331081BRJ
CSB-EP331081BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Proton glutamate symport protein (gltP), partial CSB-YP331082BRJ
CSB-EP331082BRJ
CSB-BP331082BRJ
CSB-MP331082BRJ
CSB-EP331082BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Beta-lactamase (penP) CSB-YP331083BRJ
CSB-EP331083BRJ
CSB-BP331083BRJ
CSB-MP331083BRJ
CSB-EP331083BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli D-galactarate dehydratase (garD) CSB-YP331084ENV
CSB-EP331084ENV
CSB-BP331084ENV
CSB-MP331084ENV
CSB-EP331084ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli High-affinity gluconate transporter (gntT), partial CSB-YP331085ENV
CSB-EP331085ENV
CSB-BP331085ENV
CSB-MP331085ENV
CSB-EP331085ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Multidrug-efflux transporter 2 regulator (bltR) CSB-YP331086BRJ
CSB-EP331086BRJ
CSB-BP331086BRJ
CSB-MP331086BRJ
CSB-EP331086BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Beta-N-acetylglucosaminidase (lytD), partial CSB-YP331087BRJ
CSB-EP331087BRJ
CSB-BP331087BRJ
CSB-MP331087BRJ
CSB-EP331087BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業產品。重組蛋白的技術資源在重組蛋白的開發過程中,除了表達系統之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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