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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pseudomonas sp. Phenol hydroxylase P4 protein (dmpO) CSB-YP322558PVK
CSB-EP322558PVK
CSB-BP322558PVK
CSB-MP322558PVK
CSB-EP322558PVK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human coronavirus 229E Envelope small membrane protein (E), partial CSB-YP322559HIT1
CSB-EP322559HIT1
CSB-BP322559HIT1
CSB-MP322559HIT1
CSB-EP322559HIT1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized 5.3 kDa protein CSB-YP322561ENL
CSB-EP322561ENL
CSB-BP322561ENL
CSB-MP322561ENL
CSB-EP322561ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Insertion element IS1 protein insB (insB1) CSB-YP322562SZB
CSB-EP322562SZB
CSB-BP322562SZB
CSB-MP322562SZB
CSB-EP322562SZB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative transposase InsK for insertion sequence element IS150 (insK) CSB-YP322563ENV
CSB-EP322563ENV
CSB-BP322563ENV
CSB-MP322563ENV
CSB-EP322563ENV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Insertion element IS2 uncharacterized 16.4 kDa protein CSB-YP322564ENL
CSB-EP322564ENL
CSB-BP322564ENL
CSB-MP322564ENL
CSB-EP322564ENL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Dihydrodipicolinate synthase (dapA) CSB-YP322565DWY
CSB-EP322565DWY
CSB-BP322565DWY
CSB-MP322565DWY
CSB-EP322565DWY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Lipopolysaccharide 1,2-glucosyltransferase (rfaJ) CSB-YP322566SXB
CSB-EP322566SXB
CSB-BP322566SXB
CSB-MP322566SXB
CSB-EP322566SXB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acyl-CoA reductase (luxC) CSB-YP322567PIF
CSB-EP322567PIF
CSB-BP322567PIF
CSB-MP322567PIF
CSB-EP322567PIF-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Substance P CSB-YP322568CH
CSB-EP322568CH
CSB-BP322568CH
CSB-MP322568CH
CSB-EP322568CH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leiurus quinquestriatus quinquestriatus Beta-insect depressant toxin LqqIT2 CSB-YP322569LDT
CSB-EP322569LDT
CSB-BP322569LDT
CSB-MP322569LDT
CSB-EP322569LDT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Phytochrome A (PHYA1), partial CSB-YP322570ZAX
CSB-EP322570ZAX
CSB-BP322570ZAX
CSB-MP322570ZAX
CSB-EP322570ZAX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Tryptophan synthase beta chain (trpB) CSB-YP322571GFM
CSB-EP322571GFM
CSB-BP322571GFM
CSB-MP322571GFM
CSB-EP322571GFM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Heat shock protein 60, mitochondrial (HSP60), partial CSB-BP322572SVG
CSB-MP322572SVG
CSB-EP322572SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type-2 restriction enzyme BanI (banIR) CSB-YP322573BAF
CSB-EP322573BAF
CSB-BP322573BAF
CSB-MP322573BAF
CSB-EP322573BAF-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Viral transcription factor IE2 (UL122), partial CSB-YP322574HWV
CSB-BP322574HWV
CSB-MP322574HWV
CSB-EP322574HWV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage M2 DNA terminal protein (3) CSB-YP322575BAY
CSB-EP322575BAY
CSB-BP322575BAY
CSB-MP322575BAY
CSB-EP322575BAY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage fr Lysis protein CSB-YP322576ECK
CSB-EP322576ECK
CSB-BP322576ECK
CSB-MP322576ECK
CSB-EP322576ECK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Immunoglobulin G-binding protein G (spg), partial CSB-YP322577SND
CSB-EP322577SND
CSB-BP322577SND
CSB-MP322577SND
CSB-EP322577SND-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carbon monoxide dehydrogenase medium chain (cutM) CSB-YP322578PUE
CSB-EP322578PUE
CSB-BP322578PUE
CSB-MP322578PUE
CSB-EP322578PUE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務(wù)，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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