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重組蛋白

產品名稱 貨號 規格
Recombinant Cryptococcus neoformans var. neoformans serotype D Sulfate adenylyltransferase (MET3), partial CSB-YP316128CTL
CSB-EP316128CTL
CSB-BP316128CTL
CSB-MP316128CTL
CSB-EP316128CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein YOP1 (YOP1), partial CSB-YP316129CTL1
CSB-EP316129CTL1
CSB-BP316129CTL1
CSB-MP316129CTL1
CSB-EP316129CTL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D rRNA-processing protein EFG1 (EFG1) CSB-YP316130CTK
CSB-EP316130CTK
CSB-BP316130CTK
CSB-MP316130CTK
CSB-EP316130CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Exocyst complex component EXO84 (EXO84), partial CSB-YP316131CTL
CSB-EP316131CTL
CSB-BP316131CTL
CSB-MP316131CTL
CSB-EP316131CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Hsp70 nucleotide exchange factor FES1 (FES1) CSB-YP316132CTL
CSB-EP316132CTL
CSB-BP316132CTL
CSB-MP316132CTL
CSB-EP316132CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Endoplasmic reticulum vesicle protein 25 (ERV25), partial CSB-YP316133CTK
CSB-EP316133CTK
CSB-BP316133CTK
CSB-MP316133CTK
CSB-EP316133CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D UDP-N-acetylglucosamine transferase subunit ALG13 (ALG13) CSB-YP316134CTL
CSB-EP316134CTL
CSB-BP316134CTL
CSB-MP316134CTL
CSB-EP316134CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Histone H3 (HHT1) CSB-YP316135CTL
CSB-EP316135CTL
CSB-BP316135CTL
CSB-MP316135CTL
CSB-EP316135CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-mRNA-splicing factor CLF1 (CLF1), partial CSB-YP316136CTK
CSB-EP316136CTK
CSB-BP316136CTK
CSB-MP316136CTK
CSB-EP316136CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Helicase SWR1 (SWR1), partial CSB-YP316137CTL
CSB-EP316137CTL
CSB-BP316137CTL
CSB-MP316137CTL
CSB-EP316137CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D JmjC domain-containing histone demethylation protein 1 (JHD1), partial CSB-YP316138CTL
CSB-EP316138CTL
CSB-BP316138CTL
CSB-MP316138CTL
CSB-EP316138CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Autophagy-related protein 8 (ATG8) CSB-YP316139CTK
CSB-EP316139CTK
CSB-BP316139CTK
CSB-MP316139CTK
CSB-EP316139CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Putative lipase ATG15 (ATG15), partial CSB-YP316140CTK
CSB-EP316140CTK
CSB-BP316140CTK
CSB-MP316140CTK
CSB-EP316140CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Vacuolar fusion protein MON1 (MON1), partial CSB-YP316141CTL
CSB-EP316141CTL
CSB-BP316141CTL
CSB-MP316141CTL
CSB-EP316141CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Cytosolic Fe-S cluster assembly factor CFD1 (CFD1) CSB-YP316142CTL
CSB-EP316142CTL
CSB-BP316142CTL
CSB-MP316142CTL
CSB-EP316142CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Pre-mRNA-splicing factor CEF1 (CEF1), partial CSB-YP316143CTL
CSB-EP316143CTL
CSB-BP316143CTL
CSB-MP316143CTL
CSB-EP316143CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Chromatin modification-related protein EAF1 (EAF1), partial CSB-YP316144CTK
CSB-EP316144CTK
CSB-BP316144CTK
CSB-MP316144CTK
CSB-EP316144CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Cytosolic Fe-S cluster assembly factor NAR1 (NAR1), partial CSB-YP316145CTK
CSB-EP316145CTK
CSB-BP316145CTK
CSB-MP316145CTK
CSB-EP316145CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Probable kinetochore protein NDC80 (NDC80), partial CSB-YP316146CTK
CSB-EP316146CTK
CSB-BP316146CTK
CSB-MP316146CTK
CSB-EP316146CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Clampless protein 1 (CNBB4530) CSB-YP316147CTK
CSB-EP316147CTK
CSB-BP316147CTK
CSB-MP316147CTK
CSB-EP316147CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業產品。重組蛋白的技術資源在重組蛋白的開發過程中,除了表達系統之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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