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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Probable D-lyxose ketol-isomerase (ydaE) CSB-YP310218BRJ
CSB-EP310218BRJ
CSB-BP310218BRJ
CSB-MP310218BRJ
CSB-EP310218BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized glycosyltransferase ydaM (ydaM), partial CSB-YP310219BRJ1
CSB-EP310219BRJ1
CSB-BP310219BRJ1
CSB-MP310219BRJ1
CSB-EP310219BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydbA (ydbA) CSB-YP310220BRJ
CSB-EP310220BRJ
CSB-BP310220BRJ
CSB-MP310220BRJ
CSB-EP310220BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein ydbK (ydbK), partial CSB-YP310221BRJ1
CSB-EP310221BRJ1
CSB-BP310221BRJ1
CSB-MP310221BRJ1
CSB-EP310221BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP310222BRJ
CSB-EP310222BRJ
CSB-BP310222BRJ
CSB-MP310222BRJ
CSB-EP310222BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0310 protein ydcG (ydcG) CSB-YP310223BRJ
CSB-EP310223BRJ
CSB-BP310223BRJ
CSB-MP310223BRJ
CSB-EP310223BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydcO (ydcO) CSB-YP310224BRJ
CSB-EP310224BRJ
CSB-BP310224BRJ
CSB-MP310224BRJ
CSB-EP310224BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydcT (ydcT) CSB-YP310225BRJ
CSB-EP310225BRJ
CSB-BP310225BRJ
CSB-MP310225BRJ
CSB-EP310225BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yddE (yddE), partial CSB-YP310226BRJ
CSB-EP310226BRJ
CSB-BP310226BRJ
CSB-MP310226BRJ
CSB-EP310226BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yddJ (yddJ) CSB-YP310227BRJ
CSB-EP310227BRJ
CSB-BP310227BRJ
CSB-MP310227BRJ
CSB-EP310227BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis HTH-type transcriptional regulator lrpB (lrpB) CSB-YP310228BRJ
CSB-EP310228BRJ
CSB-BP310228BRJ
CSB-MP310228BRJ
CSB-EP310228BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydeI (ydeI) CSB-YP310229BRJ
CSB-EP310229BRJ
CSB-BP310229BRJ
CSB-MP310229BRJ
CSB-EP310229BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized lipoprotein ydeJ (ydeJ) CSB-YP310230BRJ
CSB-EP310230BRJ
CSB-BP310230BRJ
CSB-MP310230BRJ
CSB-EP310230BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ydeP (ydeP) CSB-YP310231BRJ
CSB-EP310231BRJ
CSB-BP310231BRJ
CSB-MP310231BRJ
CSB-EP310231BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MFS-type transporter ydeR (ydeR), partial CSB-YP310232BRJ
CSB-EP310232BRJ
CSB-BP310232BRJ
CSB-MP310232BRJ
CSB-EP310232BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized transporter ydfC (ydfC), partial CSB-YP310233BRJ
CSB-EP310233BRJ
CSB-BP310233BRJ
CSB-MP310233BRJ
CSB-EP310233BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative ring-cleaving dioxygenase mhqO (mhqO) CSB-YP310234BRJ
CSB-EP310234BRJ
CSB-BP310234BRJ
CSB-MP310234BRJ
CSB-EP310234BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydgB (ydgB) CSB-YP310235BRJ
CSB-EP310235BRJ
CSB-BP310235BRJ
CSB-MP310235BRJ
CSB-EP310235BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative membrane protein ydgH (ydgH), partial CSB-YP310236BRJ
CSB-EP310236BRJ
CSB-BP310236BRJ
CSB-MP310236BRJ
CSB-EP310236BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UDP-glucose 6-dehydrogenase ywqF (ywqF) CSB-YP310237BRJ
CSB-EP310237BRJ
CSB-BP310237BRJ
CSB-MP310237BRJ
CSB-EP310237BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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