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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lumbricus terrestris Ubiquitin CSB-YP306220LJN
CSB-EP306220LJN
CSB-BP306220LJN
CSB-MP306220LJN
CSB-EP306220LJN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Blaptica dubia Pyrokinin-5a CSB-YP306221BHE
CSB-EP306221BHE
CSB-BP306221BHE
CSB-MP306221BHE
CSB-EP306221BHE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phyllomedusa hypochondrialis Dermaseptin-3 CSB-YP306222PIT
CSB-EP306222PIT
CSB-BP306222PIT
CSB-MP306222PIT
CSB-EP306222PIT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phyllomedusa hypochondrialis Dermaseptin-5 CSB-YP306223PIT
CSB-EP306223PIT
CSB-BP306223PIT
CSB-MP306223PIT
CSB-EP306223PIT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gadus morhua Hemoglobin subunit alpha-1 (hba1) CSB-YP306224GER
CSB-EP306224GER
CSB-BP306224GER
CSB-MP306224GER
CSB-EP306224GER-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudoalteromonas haloplanktis Superoxide dismutase [Fe] (sodB) CSB-YP306225PTP
CSB-EP306225PTP
CSB-BP306225PTP
CSB-MP306225PTP
CSB-EP306225PTP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dromaius novaehollandiae Dromaiocalcin-1 CSB-YP306226DKU
CSB-EP306226DKU
CSB-BP306226DKU
CSB-MP306226DKU
CSB-EP306226DKU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhea americana Rheacalcin-2 CSB-YP306227RKB
CSB-EP306227RKB
CSB-BP306227RKB
CSB-MP306227RKB
CSB-EP306227RKB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus profundus Heterodisulfide reductase subunit C-like protein (hdlC) CSB-YP306228DOD
CSB-EP306228DOD
CSB-BP306228DOD
CSB-MP306228DOD
CSB-EP306228DOD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus profundus F420-non-reducing hydrogenase iron-sulfur subunit D (mvhD) CSB-YP306229DOD
CSB-EP306229DOD
CSB-BP306229DOD
CSB-MP306229DOD
CSB-EP306229DOD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus profundus F420-non-reducing hydrogenase iron-sulfur subunit G (mvhG) CSB-YP306230DOD
CSB-EP306230DOD
CSB-BP306230DOD
CSB-MP306230DOD
CSB-EP306230DOD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tityus pachyurus Beta-mammal toxin Tpa2 CSB-YP306231TEU
CSB-EP306231TEU
CSB-BP306231TEU
CSB-MP306231TEU
CSB-EP306231TEU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Dicer-like protein 4 (DCL4), partial CSB-YP306232DOA
CSB-EP306232DOA
CSB-BP306232DOA
CSB-MP306232DOA
CSB-EP306232DOA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leonia cymosa Cycloviolin-B CSB-YP306233LDX
CSB-EP306233LDX
CSB-BP306233LDX
CSB-MP306233LDX
CSB-EP306233LDX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Circulin-C CSB-YP306234CHP
CSB-EP306234CHP
CSB-BP306234CHP
CSB-MP306234CHP
CSB-EP306234CHP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Circulin-F CSB-YP306235CHP
CSB-EP306235CHP
CSB-BP306235CHP
CSB-MP306235CHP
CSB-EP306235CHP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hybanthus floribundus Cyclotide Hyfl-A CSB-YP306236HQR
CSB-EP306236HQR
CSB-BP306236HQR
CSB-MP306236HQR
CSB-EP306236HQR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pycnoscelus surinamensis Periviscerokinin-1 CSB-YP306237PXK
CSB-EP306237PXK
CSB-BP306237PXK
CSB-MP306237PXK
CSB-EP306237PXK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Perisphaeria virescens Periviscerokinin-1 CSB-YP306238PGE
CSB-EP306238PGE
CSB-BP306238PGE
CSB-MP306238PGE
CSB-EP306238PGE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Panchlora viridis Periviscerokinin-1 CSB-YP306239PAS
CSB-EP306239PAS
CSB-BP306239PAS
CSB-MP306239PAS
CSB-EP306239PAS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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