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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brassica napus Enoyl-[acyl-carrier-protein] reductase [NADH], chloroplastic CSB-YP302110BWD
CSB-EP302110BWD
CSB-BP302110BWD
CSB-MP302110BWD
CSB-EP302110BWD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis Glutamyl endopeptidase (blaSE) CSB-YP302111BQU
CSB-BP302111BQU
CSB-MP302111BQU
CSB-EP302111BQU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium bovis Alanine and proline-rich secreted protein apa (apa) CSB-YP302112MOJ
CSB-EP302112MOJ
CSB-BP302112MOJ
CSB-MP302112MOJ
CSB-EP302112MOJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Centruroides noxius Toxin Cn3 CSB-YP302113DRB
CSB-EP302113DRB
CSB-BP302113DRB
CSB-MP302113DRB
CSB-EP302113DRB-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea ATP synthase 28 kDa subunit, mitochondrial CSB-YP302114FKI
CSB-EP302114FKI
CSB-BP302114FKI
CSB-MP302114FKI
CSB-EP302114FKI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S- (hydroxymethyl)mycothiol dehydrogenase CSB-YP302115AJF
CSB-EP302115AJF
CSB-BP302115AJF
CSB-MP302115AJF
CSB-EP302115AJF-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Light-harvesting protein B800/830/1020 alpha-1 chain, partial CSB-YP302116EAQ
CSB-EP302116EAQ
CSB-BP302116EAQ
CSB-MP302116EAQ
CSB-EP302116EAQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein hrpC2 (hrpC2), partial CSB-YP302117XAK1
CSB-EP302117XAK1
CSB-BP302117XAK1
CSB-MP302117XAK1
CSB-EP302117XAK1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Theromyzon tessulatum Ovohemerythrin CSB-YP302118TDW
CSB-EP302118TDW
CSB-BP302118TDW
CSB-MP302118TDW
CSB-EP302118TDW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Atractaspis bibroni Bibrotoxin CSB-YP302119AQN
CSB-EP302119AQN
CSB-BP302119AQN
CSB-MP302119AQN
CSB-EP302119AQN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hemolysin BL-binding component (hblA), partial CSB-YP302120BQJ1
CSB-EP302120BQJ1
CSB-BP302120BQJ1
CSB-MP302120BQJ1
CSB-EP302120BQJ1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lymnaea stagnalis Lymna-DF-amide 2 CSB-YP302121LRQ
CSB-EP302121LRQ
CSB-BP302121LRQ
CSB-MP302121LRQ
CSB-EP302121LRQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter marburgensis Tetrahydromethanopterin S-methyltransferase subunit E (mtrE), partial CSB-YP302122MSQ1
CSB-EP302122MSQ1
CSB-BP302122MSQ1
CSB-MP302122MSQ1
CSB-EP302122MSQ1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Lysozyme C, non-stomach isozyme (LYS) CSB-YP302123BO
CSB-EP302123BO
CSB-BP302123BO
CSB-MP302123BO
CSB-EP302123BO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Intracellular ribonuclease LX (RNALX) CSB-YP302124SZY
CSB-EP302124SZY
CSB-BP302124SZY
CSB-MP302124SZY
CSB-EP302124SZY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica napus Napin-3 CSB-YP302125BWD
CSB-EP302125BWD
CSB-BP302125BWD
CSB-MP302125BWD
CSB-EP302125BWD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Bacteriocin plantaricin-A (plnA) CSB-YP302126LMS
CSB-BP302126LMS
CSB-MP302126LMS
CSB-EP302126LMS-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Locusta migratoria Cuticle protein 70, isoforms A and B CSB-YP302127LHY
CSB-EP302127LHY
CSB-BP302127LHY
CSB-MP302127LHY
CSB-EP302127LHY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant General stress protein 9 CSB-YP302128BRI
CSB-EP302128BRI
CSB-BP302128BRI
CSB-MP302128BRI
CSB-EP302128BRI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vitis sp. Non-specific lipid-transfer protein P3 CSB-YP302129VDR
CSB-EP302129VDR
CSB-BP302129VDR
CSB-MP302129VDR
CSB-EP302129VDR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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