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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus phage phi29 Single-stranded DNA-binding protein (5) CSB-EP654323BBC 1mg/100ug/20ug
Recombinant Human Protein phosphatase PTC7 homolog (PPTC7) CSB-EP836783HU 1mg/100ug/20ug
Recombinant Shigella flexneri Nucleoside-specific channel-forming protein tsx (tsx) CSB-EP359235SZB 1mg/100ug/20ug
Recombinant Glutamicibacter nicotianae Adenylate cyclase (cya) CSB-EP326731BLY 1mg/100ug/20ug
Recombinant Mouse Synaptotagmin-like protein 1 (Sytl1), partial CSB-EP858776MO1 1mg/100ug/20ug
Recombinant Human Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE) CSB-EP897458HU 1mg/100ug/20ug
Recombinant Rabbit Prolactin-inducible protein homolog (PIP) CSB-EP018020RB 1mg/100ug/20ug
Recombinant Vaccinia virus Protein OPG154 (OPG154), Biotinylated CSB-EP318120VAI-B 1mg/100ug/20ug
Recombinant Human RNA cytosine C(5)-methyltransferase NSUN2 (NSUN2) CSB-EP626626HU 1mg/100ug/20ug
Recombinant Mouse Complement component C8 gamma chain (C8g) CSB-EP823734MO 1mg/100ug/20ug
Recombinant Human Formin-2 (FMN2), partial CSB-EP873702HU 1mg/100ug/20ug
Recombinant Mouse Hemoglobin subunit alpha (Hba) CSB-EP010147MOa2 1mg/100ug/20ug
Recombinant Rat Latent transforming growth factor beta binding protein 4 (Ltbp4), partial CSB-EP6158RA 1mg/100ug/20ug
Recombinant Human GrpE protein homolog 1, mitochondrial (GRPEL1) CSB-EP875707HU(A4) 1mg/100ug/20ug
Recombinant Bacillus subtilis RNA-binding protein YbxF (rulS) CSB-EP343190BRJ 1mg/100ug/20ug
Recombinant Escherichia coli Periplasmic trehalase (treA) CSB-EP319553ENV 1mg/100ug/20ug
Recombinant Escherichia coli Aconitate hydratase CSB-EP6086ENR(A4) 1mg/100ug/20ug
Recombinant Escherichia coli Alpha,alpha-phosphotrehalase CSB-EP6100ENR 1mg/100ug/20ug
Recombinant Human respiratory syncytial virus Protein M2-1 (M2-1) CSB-EP6173HPN 1mg/100ug/20ug
Recombinant Human Voltage-dependent calcium channel gamma-1 subunit (CACNG1), partial CSB-EP004415HU1 1mg/100ug/20ug

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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