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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanothermus fervidus DNA-binding protein HMf-1 (hmfA) CSB-EP343755MFF 1mg/100ug/20ug
Recombinant Methanothermus fervidus DNA-binding protein HMf-1 (hmfA) CSB-BP343755MFF 1mg/100ug/20ug
Recombinant Cricetulus griseus Actin, cytoplasmic 1 (ACTB) CSB-BP344170DXU
CSB-MP344170DXU
CSB-EP344170DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Interferon alpha-G (IFNAG) CSB-BP344226BO
CSB-MP344226BO
CSB-EP344226BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Thyroxine 5-deiodinase (Dio3), partial (170:U→missing) CSB-BP344617RA
CSB-MP344617RA
CSB-EP344617RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amylomyces rouxii Chitin deacetylase CSB-EP344641AJI 1mg/100μg/20μg
Recombinant Human Putative teratocarcinoma-derived growth factor 3 (TDGF1P3) CSB-EP344744HU 1mg/100ug/20ug
Recombinant Human Putative teratocarcinoma-derived growth factor 3 (TDGF1P3), partial CSB-EP344744HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative teratocarcinoma-derived growth factor 3 (TDGF1P3) CSB-MP344744HU 1mg/100ug/20ug
Recombinant Helicobacter pylori Cytotoxicity-associated immunodominant antigen (cagA), partial CSB-BP345531HUV
CSB-MP345531HUV
CSB-EP345531HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Cathelicidin-6 (CATHL6) CSB-EP345751BO 1mg/100μg/20μg
Recombinant Bovine Cathelicidin-6 (CATHL6) CSB-BP345751BO
CSB-MP345751BO
CSB-EP345751BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Ponticulin (ponA) CSB-BP345793DKK
CSB-MP345793DKK
CSB-EP345793DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parietaria judaica Probable non-specific lipid-transfer protein 2 CSB-BP345923ESB
CSB-MP345923ESB
CSB-EP345923ESB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Heterogeneous nuclear ribonucleoprotein H2 (HNRNPH2) CSB-EP346289HU 1mg/100μg/20μg
Recombinant Blattella germanica Allergen Bla g 4 CSB-BP346597BTL
CSB-MP346597BTL
CSB-EP346597BTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mannheimia haemolytica Leukotoxin (lktA),Partial,Mammalian cell CSB-MP346605ESE 1mg(1mg*1 or 500ug*2)/500μg/100μg/50μg
Recombinant Helicobacter pylori Cytotoxicity-associated immunodominant antigen (cagA), partial CSB-EP347068HUS
CSB-BP347068HUS
CSB-MP347068HUS
CSB-EP347068HUS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli CS6 fimbrial subunit B (cssB) CSB-BP347417ENL
CSB-MP347417ENL
CSB-EP347417ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Toxocara canis 26 kDa secreted antigen (TES-26) CSB-MP347478THA
CSB-EP347478THA-B
20μg/100μg/500μg

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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