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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bombyx mori Sensory neuron membrane protein 1 (SNMP1), partial CSB-YP886975BTT
CSB-EP886975BTT
CSB-BP886975BTT
CSB-MP886975BTT
CSB-EP886975BTT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster NHP2-like protein 1 homolog (hoip) CSB-YP886976DLU
CSB-EP886976DLU
CSB-BP886976DLU
CSB-MP886976DLU
CSB-EP886976DLU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila teissieri Superoxide dismutase [Cu-Zn] (Sod) CSB-YP886977DMO
CSB-EP886977DMO
CSB-BP886977DMO
CSB-MP886977DMO
CSB-EP886977DMO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Manduca sexta ATP synthase lipid-binding protein, mitochondrial, partial CSB-YP886978MPV1
CSB-EP886978MPV1
CSB-BP886978MPV1
CSB-MP886978MPV1
CSB-EP886978MPV1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Perna viridis Metallothionein (MT1) CSB-YP886979PYH
CSB-EP886979PYH
CSB-BP886979PYH
CSB-MP886979PYH
CSB-EP886979PYH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepidoglyphus destructor Mite allergen Lep d 5 CSB-YP886980LEA
CSB-EP886980LEA
CSB-BP886980LEA
CSB-MP886980LEA
CSB-EP886980LEA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Transcription initiation factor TFIID subunit 10 (Taf10) CSB-YP886981DLU
CSB-EP886981DLU
CSB-BP886981DLU
CSB-MP886981DLU
CSB-EP886981DLU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Branchiostoma belcheri Pituitary homeobox x (Ptx) CSB-YP886982BVX
CSB-EP886982BVX
CSB-BP886982BVX
CSB-MP886982BVX
CSB-EP886982BVX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus textile Omega-conotoxin-like TxMKLT1-031 CSB-YP886983DWJ
CSB-EP886983DWJ
CSB-BP886983DWJ
CSB-MP886983DWJ
CSB-EP886983DWJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus pennaceus Mu-conotoxin-like PnMKLT1-014 CSB-YP886984CPA
CSB-EP886984CPA
CSB-BP886984CPA
CSB-MP886984CPA
CSB-EP886984CPA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Myxine glutinosa Calmodulin CSB-YP886985MWW
CSB-EP886985MWW
CSB-BP886985MWW
CSB-MP886985MWW
CSB-EP886985MWW-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease H2 subunit A (rnh-2) CSB-YP886986CXY
CSB-EP886986CXY
CSB-BP886986CXY
CSB-MP886986CXY
CSB-EP886986CXY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polistes annularis Phospholipase A1 CSB-YP886987PNAE
CSB-EP886987PNAE
CSB-BP886987PNAE
CSB-MP886987PNAE
CSB-EP886987PNAE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Discosoma striata GFP-like fluorescent chromoprotein dsFP483 CSB-YP886988DEAP
CSB-EP886988DEAP
CSB-BP886988DEAP
CSB-MP886988DEAP
CSB-EP886988DEAP-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus purpurascens Conotoxin p5a CSB-YP886989CPC
CSB-EP886989CPC
CSB-BP886989CPC
CSB-MP886989CPC
CSB-EP886989CPC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aedes aegypti 40S ribosomal protein S6 (RpS6) CSB-YP886990AXQ
CSB-EP886990AXQ
CSB-BP886990AXQ
CSB-MP886990AXQ
CSB-EP886990AXQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mastigamoeba balamuthi Histone H4 CSB-YP886991MAAE
CSB-EP886991MAAE
CSB-BP886991MAAE
CSB-MP886991MAAE
CSB-EP886991MAAE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesobuthus martensii Potassium channel toxin alpha-KTx 9.2 CSB-YP886992MRE
CSB-EP886992MRE
CSB-BP886992MRE
CSB-MP886992MRE
CSB-EP886992MRE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pierisin, partial for Pieris rapae (Cabbage white butterfly) (Small white butterfly) CSB-YP886993POAA
CSB-EP886993POAA
CSB-BP886993POAA
CSB-MP886993POAA
CSB-EP886993POAA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tachypleus tridentatus Techylectin-5A CSB-YP886994TAH
CSB-EP886994TAH
CSB-BP886994TAH
CSB-MP886994TAH
CSB-EP886994TAH-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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