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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana DNA replication licensing factor MCM3 homolog (MCM3), partial CSB-YP875487DOA
CSB-EP875487DOA
CSB-BP875487DOA
CSB-MP875487DOA
CSB-EP875487DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Inactive leucine-rich repeat receptor-like serine/threonine-protein kinase At5g24100 (At5g24100), partial CSB-YP875488DOA
CSB-EP875488DOA
CSB-BP875488DOA
CSB-MP875488DOA
CSB-EP875488DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At5g39250 (At5g39250) CSB-YP875489DOA
CSB-EP875489DOA
CSB-BP875489DOA
CSB-MP875489DOA
CSB-EP875489DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Abscisic acid receptor PYL5 (PYL5) CSB-YP875490DOA
CSB-EP875490DOA
CSB-BP875490DOA
CSB-MP875490DOA
CSB-EP875490DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g39980, chloroplastic (At5g39980), partial CSB-YP875491DOA
CSB-EP875491DOA
CSB-BP875491DOA
CSB-MP875491DOA
CSB-EP875491DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Beta-D-xylosidase 4 (BXL4), partial CSB-YP875492DOA
CSB-EP875492DOA
CSB-BP875492DOA
CSB-MP875492DOA
CSB-EP875492DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g61400 (At5g61400), partial CSB-YP875493DOA
CSB-EP875493DOA
CSB-BP875493DOA
CSB-MP875493DOA
CSB-EP875493DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor TCP6 (TCP6) CSB-YP875494DOA
CSB-EP875494DOA
CSB-BP875494DOA
CSB-MP875494DOA
CSB-EP875494DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Small ubiquitin-related modifier 2 (SUMO2) CSB-YP875495DOA
CSB-EP875495DOA
CSB-BP875495DOA
CSB-MP875495DOA
CSB-EP875495DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g61800 (PCMP-E8) CSB-YP875496DOA
CSB-EP875496DOA
CSB-BP875496DOA
CSB-MP875496DOA
CSB-EP875496DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Beta-glucosidase 31 (BGLU31), partial CSB-YP875497DOA
CSB-EP875497DOA
CSB-BP875497DOA
CSB-MP875497DOA
CSB-EP875497DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Plastidial pyruvate kinase 2 (PKP2), partial CSB-YP875498DOA
CSB-EP875498DOA
CSB-BP875498DOA
CSB-MP875498DOA
CSB-EP875498DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Notchless protein homolog (NLE1) CSB-YP875499DOA
CSB-EP875499DOA
CSB-BP875499DOA
CSB-MP875499DOA
CSB-EP875499DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GDSL esterase/lipase At5g62930 (At5g62930) CSB-YP875500DOA
CSB-EP875500DOA
CSB-BP875500DOA
CSB-MP875500DOA
CSB-EP875500DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Fasciclin-like arabinogalactan protein 1 (FLA1) CSB-YP875501DOA
CSB-EP875501DOA
CSB-BP875501DOA
CSB-MP875501DOA
CSB-EP875501DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative FBD-associated F-box protein At5g56390 (At5g56390) CSB-YP875502DOA
CSB-EP875502DOA
CSB-BP875502DOA
CSB-MP875502DOA
CSB-EP875502DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytochrome P450 85A1 (CYP85A1), partial CSB-YP875503DOA
CSB-EP875503DOA
CSB-BP875503DOA
CSB-MP875503DOA
CSB-EP875503DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g16640, mitochondrial (At5g16640) CSB-YP875504DOA
CSB-EP875504DOA
CSB-BP875504DOA
CSB-MP875504DOA
CSB-EP875504DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Oligopeptide transporter 4 (OPT4), partial CSB-YP875505DOA
CSB-EP875505DOA
CSB-BP875505DOA
CSB-MP875505DOA
CSB-EP875505DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Peroxidase 70 (PER70) CSB-YP875506DOA
CSB-EP875506DOA
CSB-BP875506DOA
CSB-MP875506DOA
CSB-EP875506DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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