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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic (NCED3), partial CSB-YP868001DOA
CSB-EP868001DOA
CSB-BP868001DOA
CSB-MP868001DOA
CSB-EP868001DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Topless-related protein 2 (TPR2), partial CSB-YP868002DOA
CSB-EP868002DOA
CSB-BP868002DOA
CSB-MP868002DOA
CSB-EP868002DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Histone chaperone ASF1B (ASF1B) CSB-YP868003DOA
CSB-EP868003DOA
CSB-BP868003DOA
CSB-MP868003DOA
CSB-EP868003DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor GTE12 (GTE12) CSB-YP868004DOA
CSB-EP868004DOA
CSB-BP868004DOA
CSB-MP868004DOA
CSB-EP868004DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At3g18320 (At3g18320) CSB-YP868005DOA
CSB-EP868005DOA
CSB-BP868005DOA
CSB-MP868005DOA
CSB-EP868005DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative proline-rich receptor-like protein kinase PERK6 (PERK6), partial CSB-YP868006DOA
CSB-EP868006DOA
CSB-BP868006DOA
CSB-MP868006DOA
CSB-EP868006DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor TCP20 (TCP20) CSB-YP868007DOA
CSB-EP868007DOA
CSB-BP868007DOA
CSB-MP868007DOA
CSB-EP868007DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g25210, mitochondrial (At3g25210), partial CSB-YP868008DOA
CSB-EP868008DOA
CSB-BP868008DOA
CSB-MP868008DOA
CSB-EP868008DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter B family member 18 (ABCB18), partial CSB-YP868009DOA
CSB-EP868009DOA
CSB-BP868009DOA
CSB-MP868009DOA
CSB-EP868009DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor bHLH147 (BHLH147) CSB-YP868010DOA
CSB-EP868010DOA
CSB-BP868010DOA
CSB-MP868010DOA
CSB-EP868010DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana B3 domain-containing protein At3g17010 (At3g17010) CSB-YP868011DOA
CSB-EP868011DOA
CSB-BP868011DOA
CSB-MP868011DOA
CSB-EP868011DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor bHLH82 (BHLH82) CSB-YP868012DOA
CSB-EP868012DOA
CSB-BP868012DOA
CSB-MP868012DOA
CSB-EP868012DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative pumilio homolog 19 (APUM19) CSB-YP868013DOA
CSB-EP868013DOA
CSB-BP868013DOA
CSB-MP868013DOA
CSB-EP868013DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Serine carboxypeptidase-like 21 (SCPL21) CSB-YP868014DOA
CSB-EP868014DOA
CSB-BP868014DOA
CSB-MP868014DOA
CSB-EP868014DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana SKP1-like protein 8 (ASK8) CSB-YP868015DOA
CSB-EP868015DOA
CSB-BP868015DOA
CSB-MP868015DOA
CSB-EP868015DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Peroxidase 30 (PER30) CSB-YP868016DOA
CSB-EP868016DOA
CSB-BP868016DOA
CSB-MP868016DOA
CSB-EP868016DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana BTB/POZ domain-containing protein At3g19850 (At3g19850), partial CSB-YP868017DOA
CSB-EP868017DOA
CSB-BP868017DOA
CSB-MP868017DOA
CSB-EP868017DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable cysteine proteinase At3g19400 (At3g19400), partial CSB-YP868018DOA
CSB-EP868018DOA
CSB-BP868018DOA
CSB-MP868018DOA
CSB-EP868018DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ERF095 (ERF095) CSB-YP868019DOA
CSB-EP868019DOA
CSB-BP868019DOA
CSB-MP868019DOA
CSB-EP868019DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein PAM68-like (At5g52780), partial CSB-YP868020DOA1
CSB-EP868020DOA1
CSB-BP868020DOA1
CSB-MP868020DOA1
CSB-EP868020DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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