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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cucumis sativus Ethylene receptor 1 (ETR1), partial CSB-YP865969CXP1
CSB-EP865969CXP1
CSB-BP865969CXP1
CSB-MP865969CXP1
CSB-EP865969CXP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Profilin-2 (PRO2) CSB-YP865970NHE
CSB-EP865970NHE
CSB-BP865970NHE
CSB-MP865970NHE
CSB-EP865970NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative disease resistance RPP13-like protein 3 (RPP13L3), partial CSB-YP865971DOA
CSB-EP865971DOA
CSB-BP865971DOA
CSB-MP865971DOA
CSB-EP865971DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Deoxyuridine 5'-triphosphate nucleotidohydrolase (DUT) CSB-YP865972DOA
CSB-EP865972DOA
CSB-BP865972DOA
CSB-MP865972DOA
CSB-EP865972DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Zinc finger A20 and AN1 domain-containing stress-associated protein 10 (SAP10) CSB-YP865973DOA
CSB-EP865973DOA
CSB-BP865973DOA
CSB-MP865973DOA
CSB-EP865973DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytochrome P450 71A21 (CYP71A21), partial CSB-YP865974DOA
CSB-EP865974DOA
CSB-BP865974DOA
CSB-MP865974DOA
CSB-EP865974DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative transcription elongation factor SPT5 homolog 1 (At4g08350), partial CSB-YP865975DOA
CSB-EP865975DOA
CSB-BP865975DOA
CSB-MP865975DOA
CSB-EP865975DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Arabinogalactan peptide 13 (AGP13) CSB-YP865976DOA
CSB-EP865976DOA
CSB-BP865976DOA
CSB-MP865976DOA
CSB-EP865976DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative pentatricopeptide repeat-containing protein At3g47840 (PCMP-E43), partial CSB-YP865977DOA
CSB-EP865977DOA
CSB-BP865977DOA
CSB-MP865977DOA
CSB-EP865977DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter A family member 4 (ABCA4), partial CSB-YP865978DOA
CSB-EP865978DOA
CSB-BP865978DOA
CSB-MP865978DOA
CSB-EP865978DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Heat shock 70 kDa protein 6, chloroplastic (HSP70-6), partial CSB-YP865979DOA
CSB-EP865979DOA
CSB-BP865979DOA
CSB-MP865979DOA
CSB-EP865979DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Phosphoinositide phospholipase C 8 (PLC8), partial CSB-YP865980DOA
CSB-EP865980DOA
CSB-BP865980DOA
CSB-MP865980DOA
CSB-EP865980DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription initiation factor IIF subunit alpha (RAP74), partial CSB-YP865981DOA
CSB-EP865981DOA
CSB-BP865981DOA
CSB-MP865981DOA
CSB-EP865981DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable cyclic nucleotide-gated ion channel 16 (CNGC16), partial CSB-YP865982DOA1
CSB-EP865982DOA1
CSB-BP865982DOA1
CSB-MP865982DOA1
CSB-EP865982DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Calcineurin B-like protein 7 (CBL7) CSB-YP865983DOA
CSB-EP865983DOA
CSB-BP865983DOA
CSB-MP865983DOA
CSB-EP865983DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative protein phosphatase 2C 53 (At4g08260) CSB-YP865984DOA
CSB-EP865984DOA
CSB-BP865984DOA
CSB-MP865984DOA
CSB-EP865984DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At4g30700 (DYW9), partial CSB-YP865985DOA
CSB-EP865985DOA
CSB-BP865985DOA
CSB-MP865985DOA
CSB-EP865985DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Photosystem I reaction center subunit VI-2, chloroplastic (PSAH2), partial CSB-YP865986DOA1
CSB-EP865986DOA1
CSB-BP865986DOA1
CSB-MP865986DOA1
CSB-EP865986DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At4g30180 (At4g30180) CSB-YP865987DOA
CSB-EP865987DOA
CSB-BP865987DOA
CSB-MP865987DOA
CSB-EP865987DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor CRF2 (CRF2) CSB-YP865988DOA
CSB-EP865988DOA
CSB-BP865988DOA
CSB-MP865988DOA
CSB-EP865988DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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