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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Probable 6-phosphogluconolactonase 1 (At1g13700) CSB-YP862823DOA
CSB-EP862823DOA
CSB-BP862823DOA
CSB-MP862823DOA
CSB-EP862823DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At1g20300, mitochondrial (At1g20300), partial CSB-YP862824DOA
CSB-EP862824DOA
CSB-BP862824DOA
CSB-MP862824DOA
CSB-EP862824DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable galacturonosyltransferase-like 1 (GATL1), partial CSB-YP862825DOA
CSB-EP862825DOA
CSB-BP862825DOA
CSB-MP862825DOA
CSB-EP862825DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Expansin-A7 (EXPA7) CSB-YP862826DOA
CSB-EP862826DOA
CSB-BP862826DOA
CSB-MP862826DOA
CSB-EP862826DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor bHLH89 (BHLH89) CSB-YP862827DOA
CSB-EP862827DOA
CSB-BP862827DOA
CSB-MP862827DOA
CSB-EP862827DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pectinesterase inhibitor 1 (PMEI1) CSB-YP862828DOA
CSB-BP862828DOA
CSB-MP862828DOA
CSB-EP862828DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable cyclic nucleotide-gated ion channel 10 (CNGC10), partial CSB-YP862829DOA1
CSB-EP862829DOA1
CSB-BP862829DOA1
CSB-MP862829DOA1
CSB-EP862829DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable 26S proteasome non-ATPase regulatory subunit 3a (EMB2719) CSB-YP862830DOA
CSB-EP862830DOA
CSB-BP862830DOA
CSB-MP862830DOA
CSB-EP862830DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At1g43980, mitochondrial (PCMP-E58), partial CSB-YP862831DOA
CSB-EP862831DOA
CSB-BP862831DOA
CSB-MP862831DOA
CSB-EP862831DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein At1g48625 (At1g48625) CSB-YP862832DOA
CSB-EP862832DOA
CSB-BP862832DOA
CSB-MP862832DOA
CSB-EP862832DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ERF014 (ERF014) CSB-YP862833DOA
CSB-EP862833DOA
CSB-BP862833DOA
CSB-MP862833DOA
CSB-EP862833DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative cytochrome c oxidase subunit 6b-like (At1g32710) CSB-YP862834DOA
CSB-EP862834DOA
CSB-BP862834DOA
CSB-MP862834DOA
CSB-EP862834DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative branched-chain-amino-acid aminotransferase 7 (BCAT7) CSB-YP862835DOA
CSB-EP862835DOA
CSB-BP862835DOA
CSB-MP862835DOA
CSB-EP862835DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pirin-like protein At1g50590 (At1g50590) CSB-YP862836DOA
CSB-EP862836DOA
CSB-BP862836DOA
CSB-MP862836DOA
CSB-EP862836DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Eukaryotic peptide chain release factor subunit 1-2 (ERF1-2) CSB-YP862837DOA
CSB-EP862837DOA
CSB-BP862837DOA
CSB-MP862837DOA
CSB-EP862837DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/kelch-repeat protein At1g12870 (At1g12870) CSB-YP862838DOA
CSB-EP862838DOA
CSB-BP862838DOA
CSB-MP862838DOA
CSB-EP862838DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana G-type lectin S-receptor-like serine/threonine-protein kinase At1g11410 (At1g11410), partial CSB-YP862839DOA
CSB-EP862839DOA
CSB-BP862839DOA
CSB-MP862839DOA
CSB-EP862839DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor bHLH125 (BHLH125) CSB-YP862840DOA
CSB-EP862840DOA
CSB-BP862840DOA
CSB-MP862840DOA
CSB-EP862840DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Terpenoid synthase 22 (TPS22), partial CSB-YP862841DOA
CSB-EP862841DOA
CSB-BP862841DOA
CSB-MP862841DOA
CSB-EP862841DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glutathione S-transferase U15 (GSTU15) CSB-YP862842DOA
CSB-EP862842DOA
CSB-BP862842DOA
CSB-MP862842DOA
CSB-EP862842DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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