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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xanthomonas campestris pv. campestris Tryptophan 2,3-dioxygenase-like protein (XCC1210) CSB-YP851086XAY
CSB-EP851086XAY
CSB-BP851086XAY
CSB-MP851086XAY
CSB-EP851086XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 30S ribosomal protein S16 (rpsP) CSB-YP851087XAY
CSB-EP851087XAY
CSB-BP851087XAY
CSB-MP851087XAY
CSB-EP851087XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 50S ribosomal protein L27 (rpmA) CSB-YP851088XAY
CSB-EP851088XAY
CSB-BP851088XAY
CSB-MP851088XAY
CSB-EP851088XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Adenylosuccinate synthetase (purA) CSB-YP851089XAY
CSB-EP851089XAY
CSB-BP851089XAY
CSB-MP851089XAY
CSB-EP851089XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris UPF0133 protein XCC1002 (XCC1002) CSB-YP851090XAY
CSB-EP851090XAY
CSB-BP851090XAY
CSB-MP851090XAY
CSB-EP851090XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Peptidyl-Asp metalloendopeptidase (XCC0955) CSB-YP851091XAY
CSB-EP851091XAY
CSB-BP851091XAY
CSB-MP851091XAY
CSB-EP851091XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 50S ribosomal protein L30 (rpmD) CSB-YP851092XAY
CSB-EP851092XAY
CSB-BP851092XAY
CSB-MP851092XAY
CSB-EP851092XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 50S ribosomal protein L16 (rplP) CSB-YP851093XAY
CSB-EP851093XAY
CSB-BP851093XAY
CSB-MP851093XAY
CSB-EP851093XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 30S ribosomal protein S10 (rpsJ) CSB-YP851094XAY
CSB-EP851094XAY
CSB-BP851094XAY
CSB-MP851094XAY
CSB-EP851094XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Elongation factor Tu-A (tufA) CSB-YP851095XAY
CSB-EP851095XAY
CSB-BP851095XAY
CSB-MP851095XAY
CSB-EP851095XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Glutamyl-tRNA reductase (hemA) CSB-YP851096XAY
CSB-EP851096XAY
CSB-BP851096XAY
CSB-MP851096XAY
CSB-EP851096XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris LPS-assembly protein lptD (lptD), partial CSB-YP851097XAY
CSB-EP851097XAY
CSB-BP851097XAY
CSB-MP851097XAY
CSB-EP851097XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP851098XAY
CSB-EP851098XAY
CSB-BP851098XAY
CSB-MP851098XAY
CSB-EP851098XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris D-alanine--D-alanine ligase B (ddlB) CSB-YP851099XAY
CSB-EP851099XAY
CSB-BP851099XAY
CSB-MP851099XAY
CSB-EP851099XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP851100XAY
CSB-EP851100XAY
CSB-BP851100XAY
CSB-MP851100XAY
CSB-EP851100XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Nicotinate phosphoribosyltransferase (pncB) CSB-YP851101XAY
CSB-EP851101XAY
CSB-BP851101XAY
CSB-MP851101XAY
CSB-EP851101XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Proline--tRNA ligase (proS), partial CSB-YP851102XAY
CSB-EP851102XAY
CSB-BP851102XAY
CSB-MP851102XAY
CSB-EP851102XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris ATP synthase gamma chain (atpG) CSB-YP851103XAY
CSB-EP851103XAY
CSB-BP851103XAY
CSB-MP851103XAY
CSB-EP851103XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Phosphoribosylamine--glycine ligase (purD) CSB-YP851104XAY
CSB-EP851104XAY
CSB-BP851104XAY
CSB-MP851104XAY
CSB-EP851104XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Indole-3-glycerol phosphate synthase (trpC) CSB-YP851105XAY
CSB-EP851105XAY
CSB-BP851105XAY
CSB-MP851105XAY
CSB-EP851105XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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