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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Methanosarcina acetivorans DNA polymerase II large subunit (polC), partial CSB-YP848448MFB
CSB-EP848448MFB
CSB-BP848448MFB
CSB-MP848448MFB
CSB-EP848448MFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina acetivorans GMP synthase [glutamine-hydrolyzing] subunit B (guaAB) CSB-YP848449MFB
CSB-EP848449MFB
CSB-BP848449MFB
CSB-MP848449MFB
CSB-EP848449MFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina acetivorans Acetyl-CoA decarbonylase/synthase complex subunit alpha 3 (cdhA3), partial CSB-YP848450MFB
CSB-EP848450MFB
CSB-BP848450MFB
CSB-MP848450MFB
CSB-EP848450MFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina acetivorans Preprotein translocase subunit SecE (secE), partial CSB-YP848451MFB
CSB-EP848451MFB
CSB-BP848451MFB
CSB-MP848451MFB
CSB-EP848451MFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina acetivorans V-type ATP synthase subunit D (atpD) CSB-YP848452MFB
CSB-EP848452MFB
CSB-BP848452MFB
CSB-MP848452MFB
CSB-EP848452MFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina acetivorans Adenylosuccinate synthetase 2 (purA2) CSB-YP848453MFB
CSB-EP848453MFB
CSB-BP848453MFB
CSB-MP848453MFB
CSB-EP848453MFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina acetivorans UPF0235 protein MA_4097 (MA_4097) CSB-YP848454MFB
CSB-EP848454MFB
CSB-BP848454MFB
CSB-MP848454MFB
CSB-EP848454MFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina acetivorans DNA polymerase IV (dbh) CSB-YP848455MFB
CSB-EP848455MFB
CSB-BP848455MFB
CSB-MP848455MFB
CSB-EP848455MFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina acetivorans 50S ribosomal protein L40e (rpl40e) CSB-YP848456MFB
CSB-EP848456MFB
CSB-BP848456MFB
CSB-MP848456MFB
CSB-EP848456MFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina acetivorans Putative K (+)-stimulated pyrophosphate-energized sodium pump (hppA1), partial CSB-YP848457MFB
CSB-EP848457MFB
CSB-BP848457MFB
CSB-MP848457MFB
CSB-EP848457MFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina acetivorans 5,10-methylenetetrahydromethanopterin reductase (mer) CSB-YP848458MFB
CSB-EP848458MFB
CSB-BP848458MFB
CSB-MP848458MFB
CSB-EP848458MFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina acetivorans Translation initiation factor 2 subunit gamma (eif2g) CSB-YP848459MFB
CSB-EP848459MFB
CSB-BP848459MFB
CSB-MP848459MFB
CSB-EP848459MFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina acetivorans N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP848460MFB
CSB-EP848460MFB
CSB-BP848460MFB
CSB-MP848460MFB
CSB-EP848460MFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina acetivorans Serine hydroxymethyltransferase (glyA) CSB-YP848461MFB
CSB-EP848461MFB
CSB-BP848461MFB
CSB-MP848461MFB
CSB-EP848461MFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina acetivorans UPF0145 protein MA_3383 (MA_3383) CSB-YP848462MFB
CSB-EP848462MFB
CSB-BP848462MFB
CSB-MP848462MFB
CSB-EP848462MFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina acetivorans UPF0290 protein MA_3306 (MA_3306), partial CSB-YP848463MFB1
CSB-EP848463MFB1
CSB-BP848463MFB1
CSB-MP848463MFB1
CSB-EP848463MFB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina acetivorans Ulilysin (MA_3214) CSB-YP848464MFB
CSB-EP848464MFB
CSB-BP848464MFB
CSB-MP848464MFB
CSB-EP848464MFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina acetivorans 3-isopropylmalate dehydratase large subunit 1 (leuC1) CSB-YP848465MFB
CSB-EP848465MFB
CSB-BP848465MFB
CSB-MP848465MFB
CSB-EP848465MFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina acetivorans Tryptophan synthase beta chain 1 (trpB1) CSB-YP848466MFB
CSB-EP848466MFB
CSB-BP848466MFB
CSB-MP848466MFB
CSB-EP848466MFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanosarcina acetivorans Threonine--tRNA ligase (thrS), partial CSB-YP848467MFB
CSB-EP848467MFB
CSB-BP848467MFB
CSB-MP848467MFB
CSB-EP848467MFB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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