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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xanthomonas campestris pv. campestris Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP844266XAY
CSB-EP844266XAY
CSB-BP844266XAY
CSB-MP844266XAY
CSB-EP844266XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Asparagine--tRNA ligase (asnS) CSB-YP844267XAY
CSB-EP844267XAY
CSB-BP844267XAY
CSB-MP844267XAY
CSB-EP844267XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Phosphate import ATP-binding protein PstB (pstB) CSB-YP844268XAY
CSB-EP844268XAY
CSB-BP844268XAY
CSB-MP844268XAY
CSB-EP844268XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Chaperone protein DnaK (dnaK), partial CSB-YP844269XAY
CSB-EP844269XAY
CSB-BP844269XAY
CSB-MP844269XAY
CSB-EP844269XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris [Protein-PII] uridylyltransferase (glnD), partial CSB-YP844270XAY
CSB-EP844270XAY
CSB-BP844270XAY
CSB-MP844270XAY
CSB-EP844270XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Ribonuclease HII (rnhB) CSB-YP844271XAY
CSB-EP844271XAY
CSB-BP844271XAY
CSB-MP844271XAY
CSB-EP844271XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Ribonuclease 3 (rnc) CSB-YP844272XAY
CSB-EP844272XAY
CSB-BP844272XAY
CSB-MP844272XAY
CSB-EP844272XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP844273XAY
CSB-EP844273XAY
CSB-BP844273XAY
CSB-MP844273XAY
CSB-EP844273XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Probable septum site-determining protein MinC (minC) CSB-YP844274XAY
CSB-EP844274XAY
CSB-BP844274XAY
CSB-MP844274XAY
CSB-EP844274XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris ADP-dependent (S)-NAD (P)H-hydrate dehydratase (nnrD) CSB-YP844275XAY
CSB-EP844275XAY
CSB-BP844275XAY
CSB-MP844275XAY
CSB-EP844275XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Ribonuclease H (rnhA) CSB-YP844276XAY
CSB-EP844276XAY
CSB-BP844276XAY
CSB-MP844276XAY
CSB-EP844276XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Disulfide bond formation protein B (dsbB), partial CSB-YP844277XAY1
CSB-EP844277XAY1
CSB-BP844277XAY1
CSB-MP844277XAY1
CSB-EP844277XAY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 50S ribosomal protein L5 (rplE) CSB-YP844278XAY
CSB-EP844278XAY
CSB-BP844278XAY
CSB-MP844278XAY
CSB-EP844278XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 50S ribosomal protein L23 (rplW) CSB-YP844279XAY
CSB-EP844279XAY
CSB-BP844279XAY
CSB-MP844279XAY
CSB-EP844279XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP844280XAY
CSB-EP844280XAY
CSB-BP844280XAY
CSB-MP844280XAY
CSB-EP844280XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Acylphosphatase (acyP) CSB-YP844281XAY
CSB-EP844281XAY
CSB-BP844281XAY
CSB-MP844281XAY
CSB-EP844281XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP844282XAY
CSB-EP844282XAY
CSB-BP844282XAY
CSB-MP844282XAY
CSB-EP844282XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Bis (5'-nucleosyl)-tetraphosphatase, symmetrical (apaH) CSB-YP844283XAY
CSB-EP844283XAY
CSB-BP844283XAY
CSB-MP844283XAY
CSB-EP844283XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Cell division protein FtsQ (ftsQ), partial CSB-YP844284XAY
CSB-EP844284XAY
CSB-BP844284XAY
CSB-MP844284XAY
CSB-EP844284XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Potassium-transporting ATPase C chain (kdpC), partial CSB-YP844285XAY1
CSB-EP844285XAY1
CSB-BP844285XAY1
CSB-MP844285XAY1
CSB-EP844285XAY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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