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重組蛋白

產品名稱 貨號 規格
Recombinant Listeria monocytogenes serovar 1/2a Threonine--tRNA ligase (thrS), partial CSB-YP835028LPY
CSB-EP835028LPY
CSB-BP835028LPY
CSB-MP835028LPY
CSB-EP835028LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Probable septum site-determining protein MinC (minC) CSB-YP835029LPY
CSB-EP835029LPY
CSB-BP835029LPY
CSB-MP835029LPY
CSB-EP835029LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP835030LPY
CSB-EP835030LPY
CSB-BP835030LPY
CSB-MP835030LPY
CSB-EP835030LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP835031LPY
CSB-EP835031LPY
CSB-BP835031LPY
CSB-MP835031LPY
CSB-EP835031LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP835032LPY
CSB-EP835032LPY
CSB-BP835032LPY
CSB-MP835032LPY
CSB-EP835032LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP835033LPY
CSB-EP835033LPY
CSB-BP835033LPY
CSB-MP835033LPY
CSB-EP835033LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Acylphosphatase (acyP) CSB-YP835034LPY
CSB-EP835034LPY
CSB-BP835034LPY
CSB-MP835034LPY
CSB-EP835034LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a N utilization substance protein B homolog (nusB) CSB-YP835035LPY
CSB-EP835035LPY
CSB-BP835035LPY
CSB-MP835035LPY
CSB-EP835035LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Ribosome-binding factor A (rbfA) CSB-YP835036LPY
CSB-EP835036LPY
CSB-BP835036LPY
CSB-MP835036LPY
CSB-EP835036LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Undecaprenyl pyrophosphate synthase (uppS) CSB-YP835037LPY
CSB-EP835037LPY
CSB-BP835037LPY
CSB-MP835037LPY
CSB-EP835037LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP835038LPY1
CSB-EP835038LPY1
CSB-BP835038LPY1
CSB-MP835038LPY1
CSB-EP835038LPY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Non-canonical purine NTP pyrophosphatase (lmo1239) CSB-YP835039LPY
CSB-EP835039LPY
CSB-BP835039LPY
CSB-MP835039LPY
CSB-EP835039LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP835040LPY
CSB-EP835040LPY
CSB-BP835040LPY
CSB-MP835040LPY
CSB-EP835040LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Acetate kinase 2 (ackA2) CSB-YP835041LPY
CSB-EP835041LPY
CSB-BP835041LPY
CSB-MP835041LPY
CSB-EP835041LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Peptide deformylase (def) CSB-YP835042LPY
CSB-EP835042LPY
CSB-BP835042LPY
CSB-MP835042LPY
CSB-EP835042LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a D-alanine--poly (phosphoribitol) ligase subunit 1 (dltA), partial CSB-YP835043LPY
CSB-EP835043LPY
CSB-BP835043LPY
CSB-MP835043LPY
CSB-EP835043LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP835044LPY
CSB-EP835044LPY
CSB-BP835044LPY
CSB-MP835044LPY
CSB-EP835044LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a UPF0266 membrane protein lmo0779 (lmo0779), partial CSB-YP835045LPY1
CSB-EP835045LPY1
CSB-BP835045LPY1
CSB-MP835045LPY1
CSB-EP835045LPY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP835046LPY
CSB-EP835046LPY
CSB-BP835046LPY
CSB-MP835046LPY
CSB-EP835046LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a UPF0237 protein lmo0533 (lmo0533) CSB-YP835047LPY
CSB-EP835047LPY
CSB-BP835047LPY
CSB-MP835047LPY
CSB-EP835047LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業產品。重組蛋白的技術資源在重組蛋白的開發過程中,除了表達系統之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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