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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human Guanine nucleotide-binding protein G (i) subunit alpha-3 (GNAI3) CSB-EP009591HU(A4) 1mg/100ug/20ug
Recombinant Human Carboxylesterase 3 (CES3) CSB-EP005260HU 1mg/100ug/20ug
Recombinant Human Vigilin (HDLBP), partial CSB-EP010257HU 1mg/100ug/20ug
Recombinant Human Adipolin (C1QTNF12) CSB-EP713130HU 1mg/100ug/20ug
Recombinant Human Guanine nucleotide-binding protein G (q) subunit alpha (GNAQ) CSB-EP342271HU 1mg/100ug/20ug
Recombinant Human Toll-like receptor 5 (TLR5), partial CSB-EP023604HU 1mg/100ug/20ug
Recombinant Neisseria meningitidis serogroup B Neisseria adhesin A (nadA),partial CSB-EP5161NGGb0 1mg/100ug/20ug
Recombinant Human parainfluenza 3 virus Fusion glycoprotein F0 (F), partial CSB-EP361959HPI 1mg/100ug/20ug
Recombinant Mouse Serine protease inhibitor Kazal-type 4 (Spink4) CSB-EP022577MO 1mg/100ug/20ug
Recombinant Human Na (+)/H (+) exchange regulatory cofactor NHE-RF1 (NHERF1) CSB-EP021733HU 1mg/100ug/20ug
Recombinant Mouse Neural cell adhesion molecule 1 (Ncam1), partial CSB-EP015511MO 1mg/100ug/20ug
Recombinant Human Histone deacetylase complex subunit SAP25 (SAP25) CSB-EP855067HUb0 1mg/100ug/20ug
Recombinant Mouse Complement C1q subcomponent subunit B (C1qb), Biotinylated CSB-EP003638MO-B 1mg/100ug/20ug
Recombinant Rat CCN family member 4 (Ccn4), Biotinylated CSB-EP857523RA-B 1mg/100ug/20ug
Recombinant Mouse Regenerating islet-derived protein 3-beta (Reg3b) CSB-EP333982MOe1 1mg/100ug/20ug
Recombinant Mouse Regenerating islet-derived protein 3-gamma (Reg3g), partial CSB-EP019549MOe1 1mg/100ug/20ug
Recombinant?Human?Microtubule-associated?tyrosine?carboxypeptidase?1 (MATCAP1) CSB-EP721210HU 1mg/100ug/20ug
Recombinant Pig Neurofilament light polypeptide (NEFL), partial CSB-EP015688PIe1 1mg/100ug/20ug
Recombinant Yersinia pseudotuberculosis serotype I Major outer membrane lipoprotein Lpp (lpp) CSB-EP724051YAH 1mg/100ug/20ug
Recombinant Human Serine/threonine-protein kinase RIO1 (RIOK1) CSB-EP871562HU 1mg/100ug/20ug

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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