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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ashbya gossypii Putative ATP synthase protein 8-like protein (AGL367W-A), partial CSB-YP765595DOT
CSB-EP765595DOT
CSB-BP765595DOT
CSB-MP765595DOT
CSB-EP765595DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Spindle pole body component KRE28 (KRE28) CSB-YP765596DOT
CSB-EP765596DOT
CSB-BP765596DOT
CSB-MP765596DOT
CSB-EP765596DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Sterol 3-beta-glucosyltransferase (ATG26), partial CSB-YP765597DOT
CSB-EP765597DOT
CSB-BP765597DOT
CSB-MP765597DOT
CSB-EP765597DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mannose-1-phosphate guanyltransferase (MPG1) CSB-YP765598DOT
CSB-EP765598DOT
CSB-BP765598DOT
CSB-MP765598DOT
CSB-EP765598DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Carbamoyl-phosphate synthase arginine-specific small chain (CPA1) CSB-YP765599DOT
CSB-EP765599DOT
CSB-BP765599DOT
CSB-MP765599DOT
CSB-EP765599DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii rRNA-processing protein CGR1 (CGR1) CSB-YP765600DOT
CSB-EP765600DOT
CSB-BP765600DOT
CSB-MP765600DOT
CSB-EP765600DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii D-arabinono-1,4-lactone oxidase (ALO1), partial CSB-YP765601DOT
CSB-EP765601DOT
CSB-BP765601DOT
CSB-MP765601DOT
CSB-EP765601DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial distribution and morphology protein 32 (MDM32), partial CSB-YP765602DOT1
CSB-EP765602DOT1
CSB-BP765602DOT1
CSB-MP765602DOT1
CSB-EP765602DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial zinc maintenance protein 1, mitochondrial (MZM1) CSB-YP765603DOT
CSB-EP765603DOT
CSB-BP765603DOT
CSB-MP765603DOT
CSB-EP765603DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Serine/threonine-protein phosphatase 2A activator 1 (RRD1) CSB-YP765604DOT
CSB-EP765604DOT
CSB-BP765604DOT
CSB-MP765604DOT
CSB-EP765604DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii GDP-mannose transporter (VRG4), partial CSB-YP765605DOT
CSB-EP765605DOT
CSB-BP765605DOT
CSB-MP765605DOT
CSB-EP765605DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Oxidant-induced cell-cycle arrest protein 5 (OCA5), partial CSB-YP765606DOT
CSB-EP765606DOT
CSB-BP765606DOT
CSB-MP765606DOT
CSB-EP765606DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Elongation factor 2 (EFT1), partial CSB-YP765607DOT
CSB-EP765607DOT
CSB-BP765607DOT
CSB-MP765607DOT
CSB-EP765607DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Formation of crista junctions protein 1 (FCJ1), partial CSB-YP765608DOT1
CSB-EP765608DOT1
CSB-BP765608DOT1
CSB-MP765608DOT1
CSB-EP765608DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Glucose starvation modulator protein 1 (GSM1) CSB-YP765609DOT
CSB-EP765609DOT
CSB-BP765609DOT
CSB-MP765609DOT
CSB-EP765609DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein IBD2 (IBD2) CSB-YP765610DOT
CSB-EP765610DOT
CSB-BP765610DOT
CSB-MP765610DOT
CSB-EP765610DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii tRNA-splicing endonuclease subunit SEN34 (SEN34) CSB-YP765611DOT
CSB-EP765611DOT
CSB-BP765611DOT
CSB-MP765611DOT
CSB-EP765611DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Nuclear cap-binding protein subunit 2 (CBC2) CSB-YP765612DOT
CSB-EP765612DOT
CSB-BP765612DOT
CSB-MP765612DOT
CSB-EP765612DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Oxidation resistance protein 1 (OXR1) CSB-YP765613DOT
CSB-EP765613DOT
CSB-BP765613DOT
CSB-MP765613DOT
CSB-EP765613DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii tRNA pseudouridine synthase 1 (PUS1), partial CSB-YP765614DOT
CSB-EP765614DOT
CSB-BP765614DOT
CSB-MP765614DOT
CSB-EP765614DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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