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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian 7-cyano-7-deazaguanine synthase (queC) CSB-YP764198BAAE
CSB-EP764198BAAE
CSB-BP764198BAAE
CSB-MP764198BAAE
CSB-EP764198BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0477 protein BT9727_1129 (BT9727_1129) CSB-YP764199BAAE
CSB-EP764199BAAE
CSB-BP764199BAAE
CSB-MP764199BAAE
CSB-EP764199BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0234 protein BT9727_1064 (BT9727_1064) CSB-YP764200BAAE
CSB-EP764200BAAE
CSB-BP764200BAAE
CSB-MP764200BAAE
CSB-EP764200BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian ATP-dependent helicase/deoxyribonuclease subunit B (addB), partial CSB-YP764201BAAE
CSB-EP764201BAAE
CSB-BP764201BAAE
CSB-MP764201BAAE
CSB-EP764201BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Serine-protein kinase rsbW (rsbW) CSB-YP764202BAAE
CSB-EP764202BAAE
CSB-BP764202BAAE
CSB-MP764202BAAE
CSB-EP764202BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP764203BAAE
CSB-EP764203BAAE
CSB-BP764203BAAE
CSB-MP764203BAAE
CSB-EP764203BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Thiazole synthase (thiG) CSB-YP764204BAAE
CSB-EP764204BAAE
CSB-BP764204BAAE
CSB-MP764204BAAE
CSB-EP764204BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Probable methyl-accepting chemotaxis protein BT9727_0469 (BT9727_0469), partial CSB-YP764205BAAE
CSB-EP764205BAAE
CSB-BP764205BAAE
CSB-MP764205BAAE
CSB-EP764205BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Glutamate-1-semialdehyde 2,1-aminomutase 1 (hemL1) CSB-YP764206BAAE
CSB-EP764206BAAE
CSB-BP764206BAAE
CSB-MP764206BAAE
CSB-EP764206BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian DNA topoisomerase 3 (topB), partial CSB-YP764207BAAE
CSB-EP764207BAAE
CSB-BP764207BAAE
CSB-MP764207BAAE
CSB-EP764207BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Methionine import ATP-binding protein MetN 1 (metN1) CSB-YP764208BAAE
CSB-EP764208BAAE
CSB-BP764208BAAE
CSB-MP764208BAAE
CSB-EP764208BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP764209BAAE
CSB-EP764209BAAE
CSB-BP764209BAAE
CSB-MP764209BAAE
CSB-EP764209BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP764210BAAE
CSB-EP764210BAAE
CSB-BP764210BAAE
CSB-MP764210BAAE
CSB-EP764210BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S9 (rpsI) CSB-YP764211BAAE
CSB-EP764211BAAE
CSB-BP764211BAAE
CSB-MP764211BAAE
CSB-EP764211BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Translation initiation factor IF-1 (infA) CSB-YP764212BAAE
CSB-EP764212BAAE
CSB-BP764212BAAE
CSB-MP764212BAAE
CSB-EP764212BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S5 (rpsE) CSB-YP764213BAAE
CSB-EP764213BAAE
CSB-BP764213BAAE
CSB-MP764213BAAE
CSB-EP764213BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L2 (rplB) CSB-YP764214BAAE
CSB-EP764214BAAE
CSB-BP764214BAAE
CSB-MP764214BAAE
CSB-EP764214BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP764215BAAE
CSB-EP764215BAAE
CSB-BP764215BAAE
CSB-MP764215BAAE
CSB-EP764215BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP764216BAAE
CSB-EP764216BAAE
CSB-BP764216BAAE
CSB-MP764216BAAE
CSB-EP764216BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Type III pantothenate kinase (coaX) CSB-YP764217BAAE
CSB-EP764217BAAE
CSB-BP764217BAAE
CSB-MP764217BAAE
CSB-EP764217BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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