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重組蛋白

產品名稱 貨號 規格
Recombinant Bacillus thuringiensis subsp. konkukian GMP reductase (guaC) CSB-YP760711BAAE
CSB-EP760711BAAE
CSB-BP760711BAAE
CSB-MP760711BAAE
CSB-EP760711BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Serine hydroxymethyltransferase (glyA) CSB-YP760712BAAE
CSB-EP760712BAAE
CSB-BP760712BAAE
CSB-MP760712BAAE
CSB-EP760712BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian ATP synthase subunit alpha (atpA), partial CSB-YP760713BAAE
CSB-EP760713BAAE
CSB-BP760713BAAE
CSB-MP760713BAAE
CSB-EP760713BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP760714BAAE1
CSB-EP760714BAAE1
CSB-BP760714BAAE1
CSB-MP760714BAAE1
CSB-EP760714BAAE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UvrABC system protein B (uvrB), partial CSB-YP760715BAAE
CSB-EP760715BAAE
CSB-BP760715BAAE
CSB-MP760715BAAE
CSB-EP760715BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0042 nucleotide-binding protein BT9727_4833 (BT9727_4833) CSB-YP760716BAAE
CSB-EP760716BAAE
CSB-BP760716BAAE
CSB-MP760716BAAE
CSB-EP760716BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian SsrA-binding protein (smpB) CSB-YP760717BAAE
CSB-EP760717BAAE
CSB-BP760717BAAE
CSB-MP760717BAAE
CSB-EP760717BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Tyrosine--tRNA ligase 2 (tyrS2) CSB-YP760718BAAE
CSB-EP760718BAAE
CSB-BP760718BAAE
CSB-MP760718BAAE
CSB-EP760718BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian L-lactate dehydrogenase 2 (ldh2) CSB-YP760719BAAE
CSB-EP760719BAAE
CSB-BP760719BAAE
CSB-MP760719BAAE
CSB-EP760719BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian S-adenosylmethionine synthase (metK) CSB-YP760720BAAE
CSB-EP760720BAAE
CSB-BP760720BAAE
CSB-MP760720BAAE
CSB-EP760720BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S4 (rpsD) CSB-YP760721BAAE
CSB-EP760721BAAE
CSB-BP760721BAAE
CSB-MP760721BAAE
CSB-EP760721BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UPF0173 metal-dependent hydrolase BT9727_4343 (BT9727_4343) CSB-YP760722BAAE
CSB-EP760722BAAE
CSB-BP760722BAAE
CSB-MP760722BAAE
CSB-EP760722BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP760723BAAE
CSB-EP760723BAAE
CSB-BP760723BAAE
CSB-MP760723BAAE
CSB-EP760723BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Asparagine--tRNA ligase (asnS) CSB-YP760724BAAE
CSB-EP760724BAAE
CSB-BP760724BAAE
CSB-MP760724BAAE
CSB-EP760724BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Ribonuclease PH (rph) CSB-YP760725BAAE
CSB-EP760725BAAE
CSB-BP760725BAAE
CSB-MP760725BAAE
CSB-EP760725BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP760726BAAE
CSB-EP760726BAAE
CSB-BP760726BAAE
CSB-MP760726BAAE
CSB-EP760726BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP760727BAAE
CSB-EP760727BAAE
CSB-BP760727BAAE
CSB-MP760727BAAE
CSB-EP760727BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Uridine kinase (udk) CSB-YP760728BAAE
CSB-EP760728BAAE
CSB-BP760728BAAE
CSB-MP760728BAAE
CSB-EP760728BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP760729BAAE
CSB-EP760729BAAE
CSB-BP760729BAAE
CSB-MP760729BAAE
CSB-EP760729BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Ribosomal protein L11 methyltransferase (prmA) CSB-YP760730BAAE
CSB-EP760730BAAE
CSB-BP760730BAAE
CSB-MP760730BAAE
CSB-EP760730BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業產品。重組蛋白的技術資源在重組蛋白的開發過程中,除了表達系統之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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