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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ashbya gossypii DASH complex subunit HSK3 (HSK3) CSB-YP758712DOT
CSB-EP758712DOT
CSB-BP758712DOT
CSB-MP758712DOT
CSB-EP758712DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii C-5 sterol desaturase (ERG3), partial CSB-YP758713DOT1
CSB-EP758713DOT1
CSB-BP758713DOT1
CSB-MP758713DOT1
CSB-EP758713DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Actin-related protein 4 (ARP4) CSB-YP758714DOT
CSB-EP758714DOT
CSB-BP758714DOT
CSB-MP758714DOT
CSB-EP758714DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Mitochondrial import inner membrane translocase subunit TIM16 (PAM16) CSB-YP758715DOT
CSB-EP758715DOT
CSB-BP758715DOT
CSB-MP758715DOT
CSB-EP758715DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Oligosaccharide translocation protein RFT1 (RFT1), partial CSB-YP758716DOT
CSB-EP758716DOT
CSB-BP758716DOT
CSB-MP758716DOT
CSB-EP758716DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii DASH complex subunit DAD3 (DAD3) CSB-YP758717DOT
CSB-EP758717DOT
CSB-BP758717DOT
CSB-MP758717DOT
CSB-EP758717DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii PAB-dependent poly (A)-specific ribonuclease subunit PAN2 (PAN2), partial CSB-YP758718DOT
CSB-EP758718DOT
CSB-BP758718DOT
CSB-MP758718DOT
CSB-EP758718DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii ATP-dependent RNA helicase DBP3 (DBP3), partial CSB-YP758719DOT
CSB-EP758719DOT
CSB-BP758719DOT
CSB-MP758719DOT
CSB-EP758719DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii mRNA-capping enzyme subunit alpha (CEG1) CSB-YP758720DOT
CSB-EP758720DOT
CSB-BP758720DOT
CSB-MP758720DOT
CSB-EP758720DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii RuvB-like helicase 2 (RVB2) CSB-YP758721DOT
CSB-EP758721DOT
CSB-BP758721DOT
CSB-MP758721DOT
CSB-EP758721DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Autophagy protein 16 (ATG16) CSB-YP758722DOT
CSB-EP758722DOT
CSB-BP758722DOT
CSB-MP758722DOT
CSB-EP758722DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Inheritance of peroxisomes protein 2 (INP2), partial CSB-YP758723DOT1
CSB-EP758723DOT1
CSB-BP758723DOT1
CSB-MP758723DOT1
CSB-EP758723DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Zinc-regulated protein 8 (ZRG8), partial CSB-YP758724DOT
CSB-EP758724DOT
CSB-BP758724DOT
CSB-MP758724DOT
CSB-EP758724DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Palmitoyltransferase AKR1 (AKR1), partial CSB-YP758725DOT1
CSB-EP758725DOT1
CSB-BP758725DOT1
CSB-MP758725DOT1
CSB-EP758725DOT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii GMP synthase [glutamine-hydrolyzing] (GUA1), partial CSB-YP758726DOT
CSB-EP758726DOT
CSB-BP758726DOT
CSB-MP758726DOT
CSB-EP758726DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Histone-lysine N-methyltransferase, H3 lysine-79 specific (DOT1), partial CSB-YP758727DOT
CSB-EP758727DOT
CSB-BP758727DOT
CSB-MP758727DOT
CSB-EP758727DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Signal recognition particle SEC65 subunit (SEC65) CSB-YP758728DOT
CSB-EP758728DOT
CSB-BP758728DOT
CSB-MP758728DOT
CSB-EP758728DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein BCP1 (BCP1) CSB-YP758729DOT
CSB-EP758729DOT
CSB-BP758729DOT
CSB-MP758729DOT
CSB-EP758729DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii Protein OS-9 homolog (YOS9) CSB-YP758730DOT
CSB-EP758730DOT
CSB-BP758730DOT
CSB-MP758730DOT
CSB-EP758730DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ashbya gossypii 40S ribosomal protein S1 (RPS1) CSB-YP758731DOT
CSB-EP758731DOT
CSB-BP758731DOT
CSB-MP758731DOT
CSB-EP758731DOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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