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重組蛋白

產品名稱 貨號 規格
Recombinant Bacillus thuringiensis subsp. konkukian Potassium-transporting ATPase B chain (kdpB), partial CSB-YP757062BAAE1
CSB-EP757062BAAE1
CSB-BP757062BAAE1
CSB-MP757062BAAE1
CSB-EP757062BAAE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Ribose import ATP-binding protein RbsA (rbsA) CSB-YP757063BAAE
CSB-EP757063BAAE
CSB-BP757063BAAE
CSB-MP757063BAAE
CSB-EP757063BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian tRNA pseudouridine synthase A 2 (truA2) CSB-YP757064BAAE
CSB-EP757064BAAE
CSB-BP757064BAAE
CSB-MP757064BAAE
CSB-EP757064BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Hydroxyethylthiazole kinase (thiM) CSB-YP757065BAAE
CSB-EP757065BAAE
CSB-BP757065BAAE
CSB-MP757065BAAE
CSB-EP757065BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Ferredoxin--NADP reductase 1 (BT9727_0321) CSB-YP757066BAAE
CSB-EP757066BAAE
CSB-BP757066BAAE
CSB-MP757066BAAE
CSB-EP757066BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Heptaprenylglyceryl phosphate synthase (pcrB) CSB-YP757067BAAE
CSB-EP757067BAAE
CSB-BP757067BAAE
CSB-MP757067BAAE
CSB-EP757067BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Undecaprenyl-diphosphatase 1 (uppP1), partial CSB-YP757068BAAE1
CSB-EP757068BAAE1
CSB-BP757068BAAE1
CSB-MP757068BAAE1
CSB-EP757068BAAE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP757069BAAE
CSB-EP757069BAAE
CSB-BP757069BAAE
CSB-MP757069BAAE
CSB-EP757069BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L17 (rplQ) CSB-YP757070BAAE
CSB-EP757070BAAE
CSB-BP757070BAAE
CSB-MP757070BAAE
CSB-EP757070BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L15 (rplO) CSB-YP757071BAAE
CSB-EP757071BAAE
CSB-BP757071BAAE
CSB-MP757071BAAE
CSB-EP757071BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S8 (rpsH) CSB-YP757072BAAE
CSB-EP757072BAAE
CSB-BP757072BAAE
CSB-MP757072BAAE
CSB-EP757072BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S3 (rpsC) CSB-YP757073BAAE
CSB-EP757073BAAE
CSB-BP757073BAAE
CSB-MP757073BAAE
CSB-EP757073BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Elongation factor Tu (tuf) CSB-YP757074BAAE
CSB-EP757074BAAE
CSB-BP757074BAAE
CSB-MP757074BAAE
CSB-EP757074BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L7/L12 (rplL) CSB-YP757075BAAE
CSB-EP757075BAAE
CSB-BP757075BAAE
CSB-MP757075BAAE
CSB-EP757075BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Glutamate--tRNA ligase (gltX) CSB-YP757076BAAE
CSB-EP757076BAAE
CSB-BP757076BAAE
CSB-MP757076BAAE
CSB-EP757076BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Putative ATP:guanido phosphotransferase BT9727_0077 (karG) CSB-YP757077BAAE
CSB-EP757077BAAE
CSB-BP757077BAAE
CSB-MP757077BAAE
CSB-EP757077BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian tRNA (Ile)-lysidine synthase (tilS) CSB-YP757078BAAE
CSB-EP757078BAAE
CSB-BP757078BAAE
CSB-MP757078BAAE
CSB-EP757078BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Initiation-control protein yabA (BT9727_0029) CSB-YP757079BAAE
CSB-EP757079BAAE
CSB-BP757079BAAE
CSB-MP757079BAAE
CSB-EP757079BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrophosphatase ppaX (ppaX) CSB-YP757080BQE
CSB-EP757080BQE
CSB-BP757080BQE
CSB-MP757080BQE
CSB-EP757080BQE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable protein ABIL4 (Os05g0585400, LOC_Os05g50800) CSB-YP757081OFG
CSB-EP757081OFG
CSB-BP757081OFG
CSB-MP757081OFG
CSB-EP757081OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業產品。重組蛋白的技術資源在重組蛋白的開發過程中,除了表達系統之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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