重組蛋白
重組蛋白是解析生物相互作用的關(guān)鍵工具，廣泛應(yīng)用于各類研究實驗中。無論是商業(yè)化產(chǎn)品，還是為特定研究定制的蛋白，它們都在多種科學(xué)應(yīng)用中發(fā)揮著不可替代的作用。
若您需要優(yōu)質(zhì)重組蛋白——已驗證的生物活性、低內(nèi)毒素水平、高純度——來為您的研究提供可靠支持，這里正是您的理想之選。
歷經(jīng)18年積累，華美生物已構(gòu)建覆蓋原核與真核的五大蛋白質(zhì)表達系統(tǒng)。我們擁有包括桿狀病毒外膜蛋白（OMP）表達技術(shù)、膜蛋白表達技術(shù)等在內(nèi)的多項蛋白表達與純化專有技術(shù)，致力于為您提供高質(zhì)量的重組蛋白解決方案。
產(chǎn)品特點
- 覆蓋廣泛：擁有超過16,000種重組蛋白，涵蓋人類、小鼠、大鼠、兔、豚鼠、雞、猴、豬等多種物種的基因與靶點，支持跨物種比較研究。
- 表達系統(tǒng)全面：提供五大表達系統(tǒng)，包括大腸桿菌、酵母、桿狀病毒-昆蟲細(xì)胞、哺乳動物細(xì)胞以及特有的無細(xì)胞表達系統(tǒng)。該系統(tǒng)省去質(zhì)粒轉(zhuǎn)化、細(xì)胞培養(yǎng)、破碎離心等步驟，顯著提升蛋白制備效率。
- 多標(biāo)簽選擇：支持His、GST、Flag、MBP等多種標(biāo)簽，便于后續(xù)純化、檢測與功能研究。
- 多領(lǐng)域應(yīng)用：適用于抗原制備、細(xì)胞與分析、蛋白互作、藥物研發(fā)、酶活性檢測、ELISA標(biāo)準(zhǔn)品、體內(nèi)外研究、質(zhì)譜與結(jié)構(gòu)分析等廣泛場景。
- 無動物源成分：我們已建立全程無動物副產(chǎn)品接觸的重組蛋白生產(chǎn)線，確保產(chǎn)品的高純凈性與一致性，適用于對來源敏感的各類實驗。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì)，或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的重組蛋白，請查看華美生物蛋白定制服務(wù)。
通過選擇目標(biāo)名稱的首字母瀏覽產(chǎn)品。
| 產(chǎn)品名稱 | 貨號 | 規(guī)格 |
|---|---|---|
| Recombinant Haloarcula marismortui Translation initiation factor 2 subunit gamma (eif2g) | CSB-YP729391HTJ CSB-EP729391HTJ CSB-BP729391HTJ CSB-MP729391HTJ CSB-EP729391HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Haloarcula marismortui Isoleucine--tRNA ligase (ileS), partial | CSB-YP729392HTJ CSB-EP729392HTJ CSB-BP729392HTJ CSB-MP729392HTJ CSB-EP729392HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Haloarcula marismortui DNA-directed RNA polymerase subunit A' (rpoA2) | CSB-YP729393HTJ CSB-EP729393HTJ CSB-BP729393HTJ CSB-MP729393HTJ CSB-EP729393HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Haloarcula marismortui Protein CrcB homolog 1 (crcB1), partial | CSB-YP729394HTJ1 CSB-EP729394HTJ1 CSB-BP729394HTJ1 CSB-MP729394HTJ1 CSB-EP729394HTJ1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Haloarcula marismortui Thiamine-phosphate synthase (thiE) | CSB-YP729395HTJ CSB-EP729395HTJ CSB-BP729395HTJ CSB-MP729395HTJ CSB-EP729395HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Haloarcula marismortui Catalase-peroxidase 1 (katG1), partial | CSB-YP729396HTJ CSB-EP729396HTJ CSB-BP729396HTJ CSB-MP729396HTJ CSB-EP729396HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Haloarcula marismortui L-tyrosine decarboxylase (mfnA) | CSB-YP729397HTJ CSB-EP729397HTJ CSB-BP729397HTJ CSB-MP729397HTJ CSB-EP729397HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Haloarcula marismortui Glutamyl-tRNA reductase (hemA) | CSB-YP729398HTJ CSB-EP729398HTJ CSB-BP729398HTJ CSB-MP729398HTJ CSB-EP729398HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Haloarcula marismortui Phosphate import ATP-binding protein PstB 1 (pstB1) | CSB-YP729399HTJ CSB-EP729399HTJ CSB-BP729399HTJ CSB-MP729399HTJ CSB-EP729399HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Haloarcula marismortui DNA-directed RNA polymerase subunit L (rpoL) | CSB-YP729400HTJ CSB-EP729400HTJ CSB-BP729400HTJ CSB-MP729400HTJ CSB-EP729400HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Haloarcula marismortui Proteasome subunit alpha 1 (psmA1) | CSB-YP729401HTJ CSB-EP729401HTJ CSB-BP729401HTJ CSB-MP729401HTJ CSB-EP729401HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Haloarcula marismortui Bifunctional protein FolD 1 (folD1) | CSB-YP729402HTJ CSB-EP729402HTJ CSB-BP729402HTJ CSB-MP729402HTJ CSB-EP729402HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Haloarcula marismortui Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase (flpA) | CSB-YP729403HTJ CSB-EP729403HTJ CSB-BP729403HTJ CSB-MP729403HTJ CSB-EP729403HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Haloarcula marismortui Preprotein translocase subunit SecE (secE), partial | CSB-YP729404HTJ CSB-EP729404HTJ CSB-BP729404HTJ CSB-MP729404HTJ CSB-EP729404HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Haloarcula marismortui TATA-box-binding protein (tbp) | CSB-YP729405HTJ CSB-EP729405HTJ CSB-BP729405HTJ CSB-MP729405HTJ CSB-EP729405HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Haloarcula marismortui Type 2 DNA topoisomerase 6 subunit B (top6B), partial | CSB-YP729406HTJ CSB-EP729406HTJ CSB-BP729406HTJ CSB-MP729406HTJ CSB-EP729406HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Haloarcula marismortui Elongation factor 1-beta (ef1b) | CSB-YP729407HTJ CSB-EP729407HTJ CSB-BP729407HTJ CSB-MP729407HTJ CSB-EP729407HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Haloarcula marismortui Protoheme IX farnesyltransferase (ctaB), partial | CSB-YP729408HTJ CSB-EP729408HTJ CSB-BP729408HTJ CSB-MP729408HTJ CSB-EP729408HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Haloarcula marismortui Phosphonates import ATP-binding protein PhnC 1 (phnC1) | CSB-YP729409HTJ CSB-EP729409HTJ CSB-BP729409HTJ CSB-MP729409HTJ CSB-EP729409HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Haloarcula marismortui Cell division control protein 6 homolog 9 (cdc6-9) | CSB-YP729410HTJ CSB-EP729410HTJ CSB-BP729410HTJ CSB-MP729410HTJ CSB-EP729410HTJ-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。
第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條：如何選擇合適的表達載體?
表達載體是一種DNA分子，它攜帶特定的基因進入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。
根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務(wù)，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022
