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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xanthomonas campestris pv. campestris Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP706274XAF1
CSB-EP706274XAF1
CSB-BP706274XAF1
CSB-MP706274XAF1
CSB-EP706274XAF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris UPF0060 membrane protein XC_1229 (XC_1229), partial CSB-YP706275XAF1
CSB-EP706275XAF1
CSB-BP706275XAF1
CSB-MP706275XAF1
CSB-EP706275XAF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Protein TolB (tolB) CSB-YP706276XAF
CSB-EP706276XAF
CSB-BP706276XAF
CSB-MP706276XAF
CSB-EP706276XAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Probable transcriptional regulatory protein XC_1131 (XC_1131) CSB-YP706277XAF
CSB-EP706277XAF
CSB-BP706277XAF
CSB-MP706277XAF
CSB-EP706277XAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP706278XAF
CSB-EP706278XAF
CSB-BP706278XAF
CSB-MP706278XAF
CSB-EP706278XAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Guanylate kinase (gmk) CSB-YP706279XAF
CSB-EP706279XAF
CSB-BP706279XAF
CSB-MP706279XAF
CSB-EP706279XAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Thiamine-phosphate synthase (thiE) CSB-YP706280XAF
CSB-EP706280XAF
CSB-BP706280XAF
CSB-MP706280XAF
CSB-EP706280XAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP706281XAF
CSB-EP706281XAF
CSB-BP706281XAF
CSB-MP706281XAF
CSB-EP706281XAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Probable GTP-binding protein EngB (engB) CSB-YP706282XAF
CSB-EP706282XAF
CSB-BP706282XAF
CSB-MP706282XAF
CSB-EP706282XAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Octanoyltransferase (lipB) CSB-YP706283XAF
CSB-EP706283XAF
CSB-BP706283XAF
CSB-MP706283XAF
CSB-EP706283XAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Diaminopimelate epimerase (dapF) CSB-YP706284XAF
CSB-EP706284XAF
CSB-BP706284XAF
CSB-MP706284XAF
CSB-EP706284XAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Putative NADH dehydrogenase/NAD (P)H nitroreductase XC_0568 (XC_0568) CSB-YP706285XAF
CSB-EP706285XAF
CSB-BP706285XAF
CSB-MP706285XAF
CSB-EP706285XAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 60 kDa chaperonin (groL), partial CSB-YP706286XAF
CSB-EP706286XAF
CSB-BP706286XAF
CSB-MP706286XAF
CSB-EP706286XAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 50S ribosomal protein L13 (rplM) CSB-YP706287XAF
CSB-EP706287XAF
CSB-BP706287XAF
CSB-MP706287XAF
CSB-EP706287XAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 8-amino-7-oxononanoate synthase (bioF) CSB-YP706288XAF
CSB-EP706288XAF
CSB-BP706288XAF
CSB-MP706288XAF
CSB-EP706288XAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP706289XAF1
CSB-EP706289XAF1
CSB-BP706289XAF1
CSB-MP706289XAF1
CSB-EP706289XAF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (kduI) CSB-YP706290XAF
CSB-EP706290XAF
CSB-BP706290XAF
CSB-MP706290XAF
CSB-EP706290XAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Chromosomal replication initiator protein DnaA (dnaA) CSB-YP706291XAF
CSB-EP706291XAF
CSB-BP706291XAF
CSB-MP706291XAF
CSB-EP706291XAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human COBW domain-containing protein 6 (CBWD6) CSB-YP706292HU
CSB-EP706292HU
CSB-BP706292HU
CSB-MP706292HU
CSB-EP706292HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 3-ketoacyl-CoA synthase 8 (KCS8), partial CSB-YP706293DOA1
CSB-EP706293DOA1
CSB-BP706293DOA1
CSB-MP706293DOA1
CSB-EP706293DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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