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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Neosartorya fumigata Rhomboid protein 2 (rbd2), partial CSB-YP703347NGS1
CSB-EP703347NGS1
CSB-BP703347NGS1
CSB-MP703347NGS1
CSB-EP703347NGS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Probable beta-glucosidase J (bglJ), partial CSB-YP703348NGS
CSB-EP703348NGS
CSB-BP703348NGS
CSB-MP703348NGS
CSB-EP703348NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata NADPH--cytochrome P450 reductase (cprA), partial CSB-YP703349NGS
CSB-EP703349NGS
CSB-BP703349NGS
CSB-MP703349NGS
CSB-EP703349NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata ATP-dependent RNA helicase mak5 (mak5), partial CSB-YP703350NGS
CSB-EP703350NGS
CSB-BP703350NGS
CSB-MP703350NGS
CSB-EP703350NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Serine/threonine-protein phosphatase 2A activator 1 (rrd1) CSB-YP703351NGS
CSB-EP703351NGS
CSB-BP703351NGS
CSB-MP703351NGS
CSB-EP703351NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Mitochondrial Rho GTPase 1 (gem1), partial CSB-YP703352NGS
CSB-EP703352NGS
CSB-BP703352NGS
CSB-MP703352NGS
CSB-EP703352NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Probable 1,4-beta-D-glucan cellobiohydrolase A (cbhA) CSB-YP703353NGS
CSB-EP703353NGS
CSB-BP703353NGS
CSB-MP703353NGS
CSB-EP703353NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Eukaryotic translation initiation factor 3 subunit J (hcr1) CSB-YP703354NGS
CSB-EP703354NGS
CSB-BP703354NGS
CSB-MP703354NGS
CSB-EP703354NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Protein transport protein sec13 (sec13) CSB-YP703355NGS
CSB-EP703355NGS
CSB-BP703355NGS
CSB-MP703355NGS
CSB-EP703355NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Tethering factor for nuclear proteasome sts1 (sts1) CSB-YP703356NGS
CSB-EP703356NGS
CSB-BP703356NGS
CSB-MP703356NGS
CSB-EP703356NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Chromatin modification-related protein eaf1 (eaf1), partial CSB-YP703357NGS
CSB-EP703357NGS
CSB-BP703357NGS
CSB-MP703357NGS
CSB-EP703357NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Altered inheritance of mitochondria protein 31, mitochondrial (aim31), partial CSB-YP703358NGS1
CSB-EP703358NGS1
CSB-BP703358NGS1
CSB-MP703358NGS1
CSB-EP703358NGS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Probable ubiquitination network signaling protein acrB (acrB), partial CSB-YP703359NGS
CSB-EP703359NGS
CSB-BP703359NGS
CSB-MP703359NGS
CSB-EP703359NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Endoplasmic reticulum vesicle protein 25 (erv25), partial CSB-YP703360NGS
CSB-EP703360NGS
CSB-BP703360NGS
CSB-MP703360NGS
CSB-EP703360NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Probable endopolygalacturonase A (pgaA) CSB-YP703361NGS
CSB-EP703361NGS
CSB-BP703361NGS
CSB-MP703361NGS
CSB-EP703361NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Mannitol 2-dehydrogenase (AFUA_4G14450), partial CSB-YP703362NGS
CSB-EP703362NGS
CSB-BP703362NGS
CSB-MP703362NGS
CSB-EP703362NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Translationally-controlled tumor protein homolog (AFUA_1G16840) CSB-YP703363NGS
CSB-EP703363NGS
CSB-BP703363NGS
CSB-MP703363NGS
CSB-EP703363NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata tRNA-dihydrouridine (47) synthase [NAD (P) (+)] (dus3), partial CSB-YP703364NGS
CSB-EP703364NGS
CSB-BP703364NGS
CSB-MP703364NGS
CSB-EP703364NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata AP-2 complex subunit sigma (aps2) CSB-YP703365NGS
CSB-EP703365NGS
CSB-BP703365NGS
CSB-MP703365NGS
CSB-EP703365NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Mitogen-activated protein kinase hog1 (hog1) CSB-YP703366NGS
CSB-EP703366NGS
CSB-BP703366NGS
CSB-MP703366NGS
CSB-EP703366NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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