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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shigella dysenteriae serotype 1 HTH-type transcriptional regulator iscR (iscR) CSB-YP656901SGF
CSB-EP656901SGF
CSB-BP656901SGF
CSB-MP656901SGF
CSB-EP656901SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP656902SGF
CSB-EP656902SGF
CSB-BP656902SGF
CSB-MP656902SGF
CSB-EP656902SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 UPF0370 protein ypfN (ypfN), partial CSB-YP656903SGF
CSB-EP656903SGF
CSB-BP656903SGF
CSB-MP656903SGF
CSB-EP656903SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP656904SGF
CSB-EP656904SGF
CSB-BP656904SGF
CSB-MP656904SGF
CSB-EP656904SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Glucokinase (glk) CSB-YP656905SGF
CSB-EP656905SGF
CSB-BP656905SGF
CSB-MP656905SGF
CSB-EP656905SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 50S ribosomal protein L3 glutamine methyltransferase (prmB) CSB-YP656906SGF
CSB-EP656906SGF
CSB-BP656906SGF
CSB-MP656906SGF
CSB-EP656906SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 UPF0207 protein yfbR (yfbR) CSB-YP656907SGF
CSB-EP656907SGF
CSB-BP656907SGF
CSB-MP656907SGF
CSB-EP656907SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP656908SGF
CSB-EP656908SGF
CSB-BP656908SGF
CSB-MP656908SGF
CSB-EP656908SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Bifunctional polymyxin resistance protein ArnA (arnA), partial CSB-YP656909SGF
CSB-EP656909SGF
CSB-BP656909SGF
CSB-MP656909SGF
CSB-EP656909SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP656910SGF
CSB-EP656910SGF
CSB-BP656910SGF
CSB-MP656910SGF
CSB-EP656910SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP656911SGF
CSB-EP656911SGF
CSB-BP656911SGF
CSB-MP656911SGF
CSB-EP656911SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Asparagine--tRNA ligase (asnS) CSB-YP656912SGF
CSB-EP656912SGF
CSB-BP656912SGF
CSB-MP656912SGF
CSB-EP656912SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 D-tagatose-1,6-bisphosphate aldolase subunit GatY (gatY) CSB-YP656913SGF
CSB-EP656913SGF
CSB-BP656913SGF
CSB-MP656913SGF
CSB-EP656913SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP656914SGF
CSB-EP656914SGF
CSB-BP656914SGF
CSB-MP656914SGF
CSB-EP656914SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Flagellar L-ring protein (flgH) CSB-YP656915SGF
CSB-EP656915SGF
CSB-BP656915SGF
CSB-MP656915SGF
CSB-EP656915SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Lipoprotein-releasing system ATP-binding protein LolD (lolD) CSB-YP656916SGF
CSB-EP656916SGF
CSB-BP656916SGF
CSB-MP656916SGF
CSB-EP656916SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Anti-adapter protein iraM (iraM) CSB-YP656917SGF
CSB-EP656917SGF
CSB-BP656917SGF
CSB-MP656917SGF
CSB-EP656917SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Multidrug resistance protein MdtK (mdtK), partial CSB-YP656918SGF
CSB-EP656918SGF
CSB-BP656918SGF
CSB-MP656918SGF
CSB-EP656918SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Electron transport complex protein RnfD (rnfD), partial CSB-YP656919SGF
CSB-EP656919SGF
CSB-BP656919SGF
CSB-MP656919SGF
CSB-EP656919SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 50S ribosomal protein L35 (rpmI) CSB-YP656920SGF
CSB-EP656920SGF
CSB-BP656920SGF
CSB-MP656920SGF
CSB-EP656920SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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