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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shigella dysenteriae serotype 1 Shiga toxin subunit A (stxA) CSB-YP656925SGF
CSB-EP656925SGF
CSB-BP656925SGF
CSB-MP656925SGF
CSB-EP656925SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 UPF0259 membrane protein yciC (yciC), partial CSB-YP656926SGF1
CSB-EP656926SGF1
CSB-BP656926SGF1
CSB-MP656926SGF1
CSB-EP656926SGF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Peptide chain release factor 1 (prfA) CSB-YP656927SGF
CSB-EP656927SGF
CSB-BP656927SGF
CSB-MP656927SGF
CSB-EP656927SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Endo-type membrane-bound lytic murein transglycosylase A (emtA) CSB-YP656928SGF
CSB-EP656928SGF
CSB-BP656928SGF
CSB-MP656928SGF
CSB-EP656928SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (otsA) CSB-YP656929SGF
CSB-EP656929SGF
CSB-BP656929SGF
CSB-MP656929SGF
CSB-EP656929SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP656930SGF
CSB-EP656930SGF
CSB-BP656930SGF
CSB-MP656930SGF
CSB-EP656930SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 UvrABC system protein C (uvrC), partial CSB-YP656931SGF
CSB-EP656931SGF
CSB-BP656931SGF
CSB-MP656931SGF
CSB-EP656931SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Pyrimidine monooxygenase RutA (rutA) CSB-YP656932SGF
CSB-EP656932SGF
CSB-BP656932SGF
CSB-MP656932SGF
CSB-EP656932SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Ribosomal RNA large subunit methyltransferase I (rlmI) CSB-YP656933SGF
CSB-EP656933SGF
CSB-BP656933SGF
CSB-MP656933SGF
CSB-EP656933SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Elongation factor P-like protein (yeiP) CSB-YP656934SGF
CSB-EP656934SGF
CSB-BP656934SGF
CSB-MP656934SGF
CSB-EP656934SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Biotin synthase (bioB) CSB-YP656935SGF
CSB-EP656935SGF
CSB-BP656935SGF
CSB-MP656935SGF
CSB-EP656935SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 DNA protection during starvation protein (dps) CSB-YP656936SGF
CSB-EP656936SGF
CSB-BP656936SGF
CSB-MP656936SGF
CSB-EP656936SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Molybdenum import ATP-binding protein ModC (modC) CSB-YP656937SGF
CSB-EP656937SGF
CSB-BP656937SGF
CSB-MP656937SGF
CSB-EP656937SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 UPF0271 protein YbgL (ybgL) CSB-YP656938SGF
CSB-EP656938SGF
CSB-BP656938SGF
CSB-MP656938SGF
CSB-EP656938SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Leucine--tRNA ligase (leuS), partial CSB-YP656939SGF
CSB-EP656939SGF
CSB-BP656939SGF
CSB-MP656939SGF
CSB-EP656939SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Enterobactin exporter EntS (entS), partial CSB-YP656940SGF
CSB-EP656940SGF
CSB-BP656940SGF
CSB-MP656940SGF
CSB-EP656940SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Gamma-glutamyl phosphate reductase (proA) CSB-YP656941SGF
CSB-EP656941SGF
CSB-BP656941SGF
CSB-MP656941SGF
CSB-EP656941SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 tRNA 2-selenouridine synthase (selU) CSB-YP656942SGF
CSB-EP656942SGF
CSB-BP656942SGF
CSB-MP656942SGF
CSB-EP656942SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 UPF0345 protein YaiE (yaiE) CSB-YP656943SGF
CSB-EP656943SGF
CSB-BP656943SGF
CSB-MP656943SGF
CSB-EP656943SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP656944SGF
CSB-EP656944SGF
CSB-BP656944SGF
CSB-MP656944SGF
CSB-EP656944SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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