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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ralstonia metallidurans Chorismate synthase (aroC) CSB-YP636832RJG
CSB-EP636832RJG
CSB-BP636832RJG
CSB-MP636832RJG
CSB-EP636832RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Enolase 1 (eno1) CSB-YP636833RJG
CSB-EP636833RJG
CSB-BP636833RJG
CSB-MP636833RJG
CSB-EP636833RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Urease accessory protein UreG (ureG) CSB-YP636834RJG
CSB-EP636834RJG
CSB-BP636834RJG
CSB-MP636834RJG
CSB-EP636834RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP636835RJG
CSB-EP636835RJG
CSB-BP636835RJG
CSB-MP636835RJG
CSB-EP636835RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Ketol-acid reductoisomerase (ilvC) CSB-YP636836RJG
CSB-EP636836RJG
CSB-BP636836RJG
CSB-MP636836RJG
CSB-EP636836RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Methylglyoxal synthase (mgsA) CSB-YP636837RJG
CSB-EP636837RJG
CSB-BP636837RJG
CSB-MP636837RJG
CSB-EP636837RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP636838RJG
CSB-EP636838RJG
CSB-BP636838RJG
CSB-MP636838RJG
CSB-EP636838RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans 60 kDa chaperonin (groL), partial CSB-YP636839RJG
CSB-EP636839RJG
CSB-BP636839RJG
CSB-MP636839RJG
CSB-EP636839RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Probable rRNA maturation factor (Rmet_0451) CSB-YP636840RJG
CSB-EP636840RJG
CSB-BP636840RJG
CSB-MP636840RJG
CSB-EP636840RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP636841RJG
CSB-EP636841RJG
CSB-BP636841RJG
CSB-MP636841RJG
CSB-EP636841RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Aspartate--tRNA ligase (aspS), partial CSB-YP636842RJG
CSB-EP636842RJG
CSB-BP636842RJG
CSB-MP636842RJG
CSB-EP636842RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Outer-membrane lipoprotein LolB (lolB) CSB-YP636843RJG
CSB-EP636843RJG
CSB-BP636843RJG
CSB-MP636843RJG
CSB-EP636843RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans C4-dicarboxylate transport protein 1 (dctA1), partial CSB-YP636844RJG
CSB-EP636844RJG
CSB-BP636844RJG
CSB-MP636844RJG
CSB-EP636844RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Orotate phosphoribosyltransferase (pyrE) CSB-YP636845RJG
CSB-EP636845RJG
CSB-BP636845RJG
CSB-MP636845RJG
CSB-EP636845RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Biotin synthase (bioB) CSB-YP636846RJG
CSB-EP636846RJG
CSB-BP636846RJG
CSB-MP636846RJG
CSB-EP636846RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP636847RJG
CSB-EP636847RJG
CSB-BP636847RJG
CSB-MP636847RJG
CSB-EP636847RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP636848BAAH
CSB-EP636848BAAH
CSB-BP636848BAAH
CSB-MP636848BAAH
CSB-EP636848BAAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Translation initiation factor IF-2 (infB), partial CSB-YP636849BAAH
CSB-EP636849BAAH
CSB-BP636849BAAH
CSB-MP636849BAAH
CSB-EP636849BAAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Undecaprenyl-diphosphatase (uppP), partial CSB-YP636850BAAH
CSB-EP636850BAAH
CSB-BP636850BAAH
CSB-MP636850BAAH
CSB-EP636850BAAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein YjeA (yjeA) CSB-YP636851BAAH
CSB-EP636851BAAH
CSB-BP636851BAAH
CSB-MP636851BAAH
CSB-EP636851BAAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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