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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodopseudomonas palustris 50S ribosomal protein L21 (rplU) CSB-YP621706RAAG
CSB-EP621706RAAG
CSB-BP621706RAAG
CSB-MP621706RAAG
CSB-EP621706RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris ATP synthase subunit delta (atpH) CSB-YP621707RAAG
CSB-EP621707RAAG
CSB-BP621707RAAG
CSB-MP621707RAAG
CSB-EP621707RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris UPF0434 protein RPD_0454 (RPD_0454) CSB-YP621708RAAG
CSB-EP621708RAAG
CSB-BP621708RAAG
CSB-MP621708RAAG
CSB-EP621708RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris tRNA modification GTPase MnmE (mnmE) CSB-YP621709RAAG
CSB-EP621709RAAG
CSB-BP621709RAAG
CSB-MP621709RAAG
CSB-EP621709RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA) CSB-YP621710RAAG
CSB-EP621710RAAG
CSB-BP621710RAAG
CSB-MP621710RAAG
CSB-EP621710RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Chaperone protein DnaK (dnaK), partial CSB-YP621711RAAG
CSB-EP621711RAAG
CSB-BP621711RAAG
CSB-MP621711RAAG
CSB-EP621711RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Glycogen synthase (glgA) CSB-YP621712RAAG
CSB-EP621712RAAG
CSB-BP621712RAAG
CSB-MP621712RAAG
CSB-EP621712RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP621713RAAG
CSB-EP621713RAAG
CSB-BP621713RAAG
CSB-MP621713RAAG
CSB-EP621713RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Translation initiation factor IF-2 (infB), partial CSB-YP621714RAAG
CSB-EP621714RAAG
CSB-BP621714RAAG
CSB-MP621714RAAG
CSB-EP621714RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 50S ribosomal protein L35 (rpmI) CSB-YP621715RAAG
CSB-EP621715RAAG
CSB-BP621715RAAG
CSB-MP621715RAAG
CSB-EP621715RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris tRNA pseudouridine synthase A (truA) CSB-YP621716RAAG
CSB-EP621716RAAG
CSB-BP621716RAAG
CSB-MP621716RAAG
CSB-EP621716RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Acetylglutamate kinase (argB) CSB-YP621717RAAG
CSB-EP621717RAAG
CSB-BP621717RAAG
CSB-MP621717RAAG
CSB-EP621717RAAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Heme-containing CO-sensing transcriptional regulator rcoM 2 (rcoM2) CSB-YP621718BNV
CSB-EP621718BNV
CSB-BP621718BNV
CSB-MP621718BNV
CSB-EP621718BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Methionine import ATP-binding protein MetN 2 (metN2) CSB-YP621719BNV
CSB-EP621719BNV
CSB-BP621719BNV
CSB-MP621719BNV
CSB-EP621719BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP621720BNV
CSB-EP621720BNV
CSB-BP621720BNV
CSB-MP621720BNV
CSB-EP621720BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans UPF0060 membrane protein Bxeno_B1021 (Bxeno_B1021), partial CSB-YP621721BNV1
CSB-EP621721BNV1
CSB-BP621721BNV1
CSB-MP621721BNV1
CSB-EP621721BNV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Acetaldehyde dehydrogenase 3 (Bxeno_B0694) CSB-YP621722BNV
CSB-EP621722BNV
CSB-BP621722BNV
CSB-MP621722BNV
CSB-EP621722BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans GTP cyclohydrolase folE2 (folE2) CSB-YP621723BNV
CSB-EP621723BNV
CSB-BP621723BNV
CSB-MP621723BNV
CSB-EP621723BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans L-threonine 3-dehydrogenase (tdh) CSB-YP621724BNV
CSB-EP621724BNV
CSB-BP621724BNV
CSB-MP621724BNV
CSB-EP621724BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Putative pterin-4-alpha-carbinolamine dehydratase (Bxeno_A4379) CSB-YP621725BNV
CSB-EP621725BNV
CSB-BP621725BNV
CSB-MP621725BNV
CSB-EP621725BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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