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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Burkholderia xenovorans 50S ribosomal protein L20 (rplT) CSB-YP614498BNV
CSB-EP614498BNV
CSB-BP614498BNV
CSB-MP614498BNV
CSB-EP614498BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans UPF0391 membrane protein Bxeno_A2958 (Bxeno_A2958), partial CSB-YP614499BNV
CSB-EP614499BNV
CSB-BP614499BNV
CSB-MP614499BNV
CSB-EP614499BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Protein hfq (hfq) CSB-YP614500BNV
CSB-EP614500BNV
CSB-BP614500BNV
CSB-MP614500BNV
CSB-EP614500BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans DNA ligase (ligA), partial CSB-YP614501BNV
CSB-EP614501BNV
CSB-BP614501BNV
CSB-MP614501BNV
CSB-EP614501BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Lipid-A-disaccharide synthase (lpxB) CSB-YP614502BNV
CSB-EP614502BNV
CSB-BP614502BNV
CSB-MP614502BNV
CSB-EP614502BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP614503BNV
CSB-EP614503BNV
CSB-BP614503BNV
CSB-MP614503BNV
CSB-EP614503BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Phosphonoacetaldehyde hydrolase (phnX) CSB-YP614504BNV
CSB-EP614504BNV
CSB-BP614504BNV
CSB-MP614504BNV
CSB-EP614504BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Glucose-6-phosphate isomerase (pgi), partial CSB-YP614505BNV
CSB-EP614505BNV
CSB-BP614505BNV
CSB-MP614505BNV
CSB-EP614505BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Recombination protein RecR (recR) CSB-YP614506BNV
CSB-EP614506BNV
CSB-BP614506BNV
CSB-MP614506BNV
CSB-EP614506BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans 30S ribosomal protein S18 (rpsR) CSB-YP614507BNV
CSB-EP614507BNV
CSB-BP614507BNV
CSB-MP614507BNV
CSB-EP614507BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Dihydroorotate dehydrogenase (quinone) (pyrD) CSB-YP614508BNV
CSB-EP614508BNV
CSB-BP614508BNV
CSB-MP614508BNV
CSB-EP614508BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Calcium/calmodulin-dependent protein kinase type 1 (CAMK1) CSB-YP614509HU
CSB-EP614509HU
CSB-BP614509HU
CSB-MP614509HU
CSB-EP614509HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Collagen alpha-3 (IX) chain (COL9A3) CSB-YP614510HU
CSB-EP614510HU
CSB-BP614510HU
CSB-MP614510HU
CSB-EP614510HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Ribosome-binding factor A (rbfA) CSB-YP614511BNV
CSB-EP614511BNV
CSB-BP614511BNV
CSB-MP614511BNV
CSB-EP614511BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia xenovorans Ribosome maturation factor RimP (rimP) CSB-YP614512BNV
CSB-EP614512BNV
CSB-BP614512BNV
CSB-MP614512BNV
CSB-EP614512BNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Nidogen-2 (NID2), partial CSB-YP614513HU
CSB-EP614513HU
CSB-BP614513HU
CSB-MP614513HU
CSB-EP614513HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein DGCR6 (DGCR6) CSB-YP614515HU
CSB-EP614515HU
CSB-BP614515HU
CSB-MP614515HU
CSB-EP614515HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tripartite motif-containing protein 14 (TRIM14) CSB-YP614516HU
CSB-EP614516HU
CSB-BP614516HU
CSB-MP614516HU
CSB-EP614516HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human DNA polymerase alpha subunit B (POLA2) CSB-YP614517HU
CSB-EP614517HU
CSB-BP614517HU
CSB-MP614517HU
CSB-EP614517HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN (WRN), partial CSB-YP614518HU
CSB-EP614518HU
CSB-BP614518HU
CSB-MP614518HU
CSB-EP614518HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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