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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human Serine/threonine-protein kinase LATS1 (LATS1), partial CSB-EP012769HU 1mg/100ug/20ug
Recombinant Vaccinia virus Envelope phospholipase F13 (VACWR052) CSB-EP356185VAI 1mg/100ug/20ug
Recombinant Human Methyltransferase-like protein 25 (METTL25) CSB-EP839813HU 1mg/100ug/20ug
Recombinant Human N(6)-adenine-specific methyltransferase METTL4 (METTL4) CSB-EP850793HU 1mg/100ug/20ug
Recombinant Vaccinia virus 3 beta-hydroxysteroid dehydrogenase/Delta 5——》4-isomerase (VACWR170) CSB-EP340805VAI 1mg/100ug/20ug
Recombinant Variola virus Envelope protein F13 (C17L) CSB-EP330288VAR 1mg/100ug/20ug
Recombinant Amaranthus caudatus Antimicrobial peptide 2 CSB-EP340863AIH 1mg/100ug/20ug
Recombinant Human metapneumovirus Fusion glycoprotein F0 (F), partial CSB-EP751041HDAM1 1mg/100ug/20ug
Recombinant Rat Cathepsin D (Ctsd) CSB-EP006187RA 1mg/100ug/20ug
Recombinant Escherichia phage T7 T7 RNA polymerase (1), partial CSB-EP018353EEB 1mg/100ug/20ug
Recombinant Human Cyclin-A2 (CCNA2) CSB-EP004805HU 1mg/100ug/20ug
Recombinant Human Origin recognition complex subunit 1 (ORC1) CSB-EP621667HU(A4) 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human?Glycine?receptor?subunit?alpha-1 (GLRA1), partial CSB-EP009516HU 1mg/100ug/20ug
Recombinant Human Mitochondrial antiviral-signaling protein (MAVS), partial,Biotinylated CSB-EP772018HU-B 1mg/100ug/20ug
Recombinant Locusta migratoria OBP10 protein (OBP10) (I18L,V57I,N131Q) CSB-EP4487LHY(M) 1mg/100ug/20ug
Recombinant Human adenovirus F serotype 40 Hexon protein (L3), partial CSB-EP318172HIR 1mg/100ug/20ug
Recombinant Mouse Cytochrome c oxidase subunit 5A, mitochondrial (Cox5a) CSB-EP005836MO 1mg/100ug/20ug
Recombinant Escherichia coli Dihydrofolate reductase (folA) CSB-EP006847ENV 1mg/100ug/20ug
Recombinant Rat Stathmin-2 (Stmn2) CSB-EP022858RA 1mg/100ug/20ug
Recombinant Human Potassium-transporting ATPase alpha chain 1 (ATP4A), partial CSB-EP002342HU 1mg/100ug/20ug

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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