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重組蛋白

產品名稱 貨號 規格
Recombinant Lysinibacillus sphaericus Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP539147LRV
CSB-EP539147LRV
CSB-BP539147LRV
CSB-MP539147LRV
CSB-EP539147LRV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus ATP-dependent protease subunit HslV (hslV) CSB-YP539148LRV
CSB-EP539148LRV
CSB-BP539148LRV
CSB-MP539148LRV
CSB-EP539148LRV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP539149LRV
CSB-EP539149LRV
CSB-BP539149LRV
CSB-MP539149LRV
CSB-EP539149LRV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus Ribonuclease Y (rny), partial CSB-YP539150LRV
CSB-EP539150LRV
CSB-BP539150LRV
CSB-MP539150LRV
CSB-EP539150LRV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP539151LRV
CSB-EP539151LRV
CSB-BP539151LRV
CSB-MP539151LRV
CSB-EP539151LRV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus LexA repressor (lexA) CSB-YP539152LRV
CSB-EP539152LRV
CSB-BP539152LRV
CSB-MP539152LRV
CSB-EP539152LRV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP539153LRV
CSB-EP539153LRV
CSB-BP539153LRV
CSB-MP539153LRV
CSB-EP539153LRV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus N utilization substance protein B homolog (nusB) CSB-YP539154LRV
CSB-EP539154LRV
CSB-BP539154LRV
CSB-MP539154LRV
CSB-EP539154LRV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB) CSB-YP539155LRV
CSB-EP539155LRV
CSB-BP539155LRV
CSB-MP539155LRV
CSB-EP539155LRV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus Redox-sensing transcriptional repressor rex (rex) CSB-YP539156LRV
CSB-EP539156LRV
CSB-BP539156LRV
CSB-MP539156LRV
CSB-EP539156LRV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus Demethylmenaquinone methyltransferase (ubiE) CSB-YP539157LRV
CSB-EP539157LRV
CSB-BP539157LRV
CSB-MP539157LRV
CSB-EP539157LRV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus Probable endonuclease 4 (nfo) CSB-YP539158LRV
CSB-EP539158LRV
CSB-BP539158LRV
CSB-MP539158LRV
CSB-EP539158LRV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus 30S ribosomal protein S21 (rpsU) CSB-YP539159LRV
CSB-EP539159LRV
CSB-BP539159LRV
CSB-MP539159LRV
CSB-EP539159LRV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP539160LRV
CSB-EP539160LRV
CSB-BP539160LRV
CSB-MP539160LRV
CSB-EP539160LRV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus Pantothenate synthetase (panC) CSB-YP539161LRV
CSB-EP539161LRV
CSB-BP539161LRV
CSB-MP539161LRV
CSB-EP539161LRV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus Alanine--tRNA ligase (alaS), partial CSB-YP539162LRV
CSB-EP539162LRV
CSB-BP539162LRV
CSB-MP539162LRV
CSB-EP539162LRV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus Aspartate--tRNA ligase (aspS), partial CSB-YP539163LRV
CSB-EP539163LRV
CSB-BP539163LRV
CSB-MP539163LRV
CSB-EP539163LRV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus Probable septum site-determining protein MinC (minC) CSB-YP539164LRV
CSB-EP539164LRV
CSB-BP539164LRV
CSB-MP539164LRV
CSB-EP539164LRV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus Prolipoprotein diacylglyceryl transferase (lgt), partial CSB-YP539165LRV1
CSB-EP539165LRV1
CSB-BP539165LRV1
CSB-MP539165LRV1
CSB-EP539165LRV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysinibacillus sphaericus ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP539166LRV
CSB-EP539166LRV
CSB-BP539166LRV
CSB-MP539166LRV
CSB-EP539166LRV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業產品。重組蛋白的技術資源在重組蛋白的開發過程中,除了表達系統之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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