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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator yczG (yczG) CSB-YP523258BRJ
CSB-EP523258BRJ
CSB-BP523258BRJ
CSB-MP523258BRJ
CSB-EP523258BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydaL (ydaL), partial CSB-YP523259BRJ
CSB-EP523259BRJ
CSB-BP523259BRJ
CSB-MP523259BRJ
CSB-EP523259BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Phosphatase rapI inhibitor (phrI) CSB-YP523260BRJ
CSB-EP523260BRJ
CSB-BP523260BRJ
CSB-MP523260BRJ
CSB-EP523260BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis DNA ligase (ligA), partial CSB-YP523261BRJ
CSB-EP523261BRJ
CSB-BP523261BRJ
CSB-MP523261BRJ
CSB-EP523261BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative DNA methyltransferase yeeA (yeeA), partial CSB-YP523262BRJ
CSB-EP523262BRJ
CSB-BP523262BRJ
CSB-MP523262BRJ
CSB-EP523262BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yesE (yesE) CSB-YP523263BRJ
CSB-EP523263BRJ
CSB-BP523263BRJ
CSB-MP523263BRJ
CSB-EP523263BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized transcriptional regulatory protein yesN (yesN) CSB-YP523264BRJ
CSB-EP523264BRJ
CSB-BP523264BRJ
CSB-MP523264BRJ
CSB-EP523264BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis HTH-type transcriptional regulator yesS (yesS), partial CSB-YP523265BRJ
CSB-EP523265BRJ
CSB-BP523265BRJ
CSB-MP523265BRJ
CSB-EP523265BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Rhamnogalacturonan exolyase yesX (yesX), partial CSB-YP523266BRJ
CSB-EP523266BRJ
CSB-BP523266BRJ
CSB-MP523266BRJ
CSB-EP523266BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yezB (yezB) CSB-YP523267BRJ
CSB-EP523267BRJ
CSB-BP523267BRJ
CSB-MP523267BRJ
CSB-EP523267BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yfnD (yfnD) CSB-YP523268BRJ
CSB-EP523268BRJ
CSB-BP523268BRJ
CSB-MP523268BRJ
CSB-EP523268BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Small, acid-soluble spore protein H (sspH) CSB-YP523269BRJ
CSB-EP523269BRJ
CSB-BP523269BRJ
CSB-MP523269BRJ
CSB-EP523269BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yfjB (yfjB) CSB-YP523270BRJ
CSB-EP523270BRJ
CSB-BP523270BRJ
CSB-MP523270BRJ
CSB-EP523270BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative metal-dependent hydrolase yfiT (yfiT) CSB-YP523271BRJ
CSB-EP523271BRJ
CSB-BP523271BRJ
CSB-MP523271BRJ
CSB-EP523271BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yfhD (yfhD) CSB-YP523272BRJ
CSB-EP523272BRJ
CSB-BP523272BRJ
CSB-MP523272BRJ
CSB-EP523272BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MFS-type transporter yfhI (yfhI), partial CSB-YP523273BRJ
CSB-EP523273BRJ
CSB-BP523273BRJ
CSB-MP523273BRJ
CSB-EP523273BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yfhO (yfhO), partial CSB-YP523274BRJ
CSB-EP523274BRJ
CSB-BP523274BRJ
CSB-MP523274BRJ
CSB-EP523274BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Alternate 30S ribosomal protein S14 (rpsN2) CSB-YP523275BRJ
CSB-EP523275BRJ
CSB-BP523275BRJ
CSB-MP523275BRJ
CSB-EP523275BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative efflux system component yhbJ (yhbJ), partial CSB-YP523276BRJ1
CSB-EP523276BRJ1
CSB-BP523276BRJ1
CSB-MP523276BRJ1
CSB-EP523276BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative acetyltransferase YjbC (yjbC) CSB-YP523277BRJ
CSB-EP523277BRJ
CSB-BP523277BRJ
CSB-MP523277BRJ
CSB-EP523277BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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