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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Nitric oxide synthase oxygenase (nos) CSB-YP520215BRJ
CSB-EP520215BRJ
CSB-BP520215BRJ
CSB-MP520215BRJ
CSB-EP520215BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable coenzyme A transferase subunit beta (yodR) CSB-YP520216BRJ
CSB-EP520216BRJ
CSB-BP520216BRJ
CSB-MP520216BRJ
CSB-EP520216BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0118 membrane protein yrrI (yrrI), partial CSB-YP520217BRJ
CSB-EP520217BRJ
CSB-BP520217BRJ
CSB-MP520217BRJ
CSB-EP520217BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yccF (yccF) CSB-YP520218BRJ
CSB-EP520218BRJ
CSB-BP520218BRJ
CSB-MP520218BRJ
CSB-EP520218BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein yfkQ (yfkQ), partial CSB-YP520219BRJ1
CSB-EP520219BRJ1
CSB-BP520219BRJ1
CSB-MP520219BRJ1
CSB-EP520219BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yopT (yopT) CSB-YP520220BRJ
CSB-EP520220BRJ
CSB-BP520220BRJ
CSB-MP520220BRJ
CSB-EP520220BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yceB (yceB) CSB-YP520221BRJ
CSB-EP520221BRJ
CSB-BP520221BRJ
CSB-MP520221BRJ
CSB-EP520221BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis SPBc2 prophage-derived uncharacterized protein yopZ (yopZ), partial CSB-YP520222BRJ1
CSB-EP520222BRJ1
CSB-BP520222BRJ1
CSB-MP520222BRJ1
CSB-EP520222BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yocB (yocB) CSB-YP520223BRJ
CSB-EP520223BRJ
CSB-BP520223BRJ
CSB-MP520223BRJ
CSB-EP520223BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized sodium-dependent transporter yocS (yocS), partial CSB-YP520224BRJ
CSB-EP520224BRJ
CSB-BP520224BRJ
CSB-MP520224BRJ
CSB-EP520224BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Transcriptional regulatory protein CitT (citT) CSB-YP520225BRJ
CSB-EP520225BRJ
CSB-BP520225BRJ
CSB-MP520225BRJ
CSB-EP520225BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized UDP-glucosyltransferase yjiC (yjiC) CSB-YP520226BRJ
CSB-EP520226BRJ
CSB-BP520226BRJ
CSB-MP520226BRJ
CSB-EP520226BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Branched-chain amino acid transport system carrier protein BraB (braB), partial CSB-YP520227BRJ
CSB-EP520227BRJ
CSB-BP520227BRJ
CSB-MP520227BRJ
CSB-EP520227BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yjfA (yjfA) CSB-YP520228BRJ
CSB-EP520228BRJ
CSB-BP520228BRJ
CSB-MP520228BRJ
CSB-EP520228BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Unsaturated rhamnogalacturonyl hydrolase YteR (yteR) CSB-YP520229BRJ
CSB-EP520229BRJ
CSB-BP520229BRJ
CSB-MP520229BRJ
CSB-EP520229BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative spore germination protein YfkT (yfkT), partial CSB-YP520230BRJ
CSB-EP520230BRJ
CSB-BP520230BRJ
CSB-MP520230BRJ
CSB-EP520230BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis ATP-dependent DNA helicase pcrA (pcrA), partial CSB-YP520231BRJ
CSB-EP520231BRJ
CSB-BP520231BRJ
CSB-MP520231BRJ
CSB-EP520231BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ykuJ (ykuJ) CSB-YP520232BRJ
CSB-EP520232BRJ
CSB-BP520232BRJ
CSB-MP520232BRJ
CSB-EP520232BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Cytochrome c-551 (cccB) CSB-YP520233BRJ
CSB-EP520233BRJ
CSB-BP520233BRJ
CSB-MP520233BRJ
CSB-EP520233BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yobK (yobK) CSB-YP520234BRJ
CSB-EP520234BRJ
CSB-BP520234BRJ
CSB-MP520234BRJ
CSB-EP520234BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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