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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli HTH-type transcriptional regulator malT (malT), partial CSB-YP513330ENU
CSB-EP513330ENU
CSB-BP513330ENU
CSB-MP513330ENU
CSB-EP513330ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Sulfurtransferase TusA (tusA) CSB-YP513331ENU
CSB-EP513331ENU
CSB-BP513331ENU
CSB-MP513331ENU
CSB-EP513331ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein CrcB homolog (crcB), partial CSB-YP513332ENU1
CSB-EP513332ENU1
CSB-BP513332ENU1
CSB-MP513332ENU1
CSB-EP513332ENU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP513333ENU
CSB-EP513333ENU
CSB-BP513333ENU
CSB-MP513333ENU
CSB-EP513333ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Potassium-transporting ATPase C chain (kdpC), partial CSB-YP513334ENU1
CSB-EP513334ENU1
CSB-BP513334ENU1
CSB-MP513334ENU1
CSB-EP513334ENU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Tautomerase pptA (pptA) CSB-YP513335ENU
CSB-EP513335ENU
CSB-BP513335ENU
CSB-MP513335ENU
CSB-EP513335ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0257 lipoprotein ynfC (ynfC) CSB-YP513336ENU
CSB-EP513336ENU
CSB-BP513336ENU
CSB-MP513336ENU
CSB-EP513336ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP513337ENU
CSB-EP513337ENU
CSB-BP513337ENU
CSB-MP513337ENU
CSB-EP513337ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP513338ENU
CSB-EP513338ENU
CSB-BP513338ENU
CSB-MP513338ENU
CSB-EP513338ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Co-chaperone protein hscB (hscB) CSB-YP513339ENU
CSB-EP513339ENU
CSB-BP513339ENU
CSB-MP513339ENU
CSB-EP513339ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glyoxylate/hydroxypyruvate reductase B (ghrB) CSB-YP513340ENU
CSB-EP513340ENU
CSB-BP513340ENU
CSB-MP513340ENU
CSB-EP513340ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphopantetheine adenylyltransferase (coaD) CSB-YP513341ENU
CSB-EP513341ENU
CSB-BP513341ENU
CSB-MP513341ENU
CSB-EP513341ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Purine ribonucleoside efflux pump nepI (nepI), partial CSB-YP513342ENU
CSB-EP513342ENU
CSB-BP513342ENU
CSB-MP513342ENU
CSB-EP513342ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative transport protein YbjL (ybjL), partial CSB-YP513343ENU
CSB-EP513343ENU
CSB-BP513343ENU
CSB-MP513343ENU
CSB-EP513343ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Hydroxylamine reductase (hcp) CSB-YP513344ENU
CSB-EP513344ENU
CSB-BP513344ENU
CSB-MP513344ENU
CSB-EP513344ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP513345ENU
CSB-EP513345ENU
CSB-BP513345ENU
CSB-MP513345ENU
CSB-EP513345ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Quinate/shikimate dehydrogenase (ydiB) CSB-YP513346ENU
CSB-EP513346ENU
CSB-BP513346ENU
CSB-MP513346ENU
CSB-EP513346ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP513347ENU
CSB-EP513347ENU
CSB-BP513347ENU
CSB-MP513347ENU
CSB-EP513347ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosome maturation factor RimM (rimM) CSB-YP513348ENU
CSB-EP513348ENU
CSB-BP513348ENU
CSB-MP513348ENU
CSB-EP513348ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein nrdI (nrdI) CSB-YP513349ENU
CSB-EP513349ENU
CSB-BP513349ENU
CSB-MP513349ENU
CSB-EP513349ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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