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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Zygosaccharomyces rouxii Methylthioribulose-1-phosphate dehydratase (MDE1) CSB-YP509523ZBL
CSB-EP509523ZBL
CSB-BP509523ZBL
CSB-MP509523ZBL
CSB-EP509523ZBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zygosaccharomyces rouxii SWI5-dependent HO expression protein 2 (SHE2) CSB-YP509524ZBL
CSB-EP509524ZBL
CSB-BP509524ZBL
CSB-MP509524ZBL
CSB-EP509524ZBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachancea thermotolerans Protein HRI1 (HRI1) CSB-YP509525LMB
CSB-EP509525LMB
CSB-BP509525LMB
CSB-MP509525LMB
CSB-EP509525LMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lachancea thermotolerans Probable metalloreductase AIM14 (AIM14), partial CSB-YP509526LMB1
CSB-EP509526LMB1
CSB-BP509526LMB1
CSB-MP509526LMB1
CSB-EP509526LMB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zygosaccharomyces rouxii ATPase expression protein 1, mitochondrial (AEP1), partial CSB-YP509527ZBL
CSB-EP509527ZBL
CSB-BP509527ZBL
CSB-MP509527ZBL
CSB-EP509527ZBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zygosaccharomyces rouxii Mitochondrial distribution and morphology protein 34 (MDM34), partial CSB-YP509528ZBL
CSB-EP509528ZBL
CSB-BP509528ZBL
CSB-MP509528ZBL
CSB-EP509528ZBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae ATPase GET3 (GET3) CSB-YP509529DOP
CSB-EP509529DOP
CSB-BP509529DOP
CSB-MP509529DOP
CSB-EP509529DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Methionine aminopeptidase 2 homolog MCYG_01318 (MCYG_01318) CSB-YP509530DOP
CSB-EP509530DOP
CSB-BP509530DOP
CSB-MP509530DOP
CSB-EP509530DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Metallocarboxypeptidase A-like protein MCYG_01475 (MCYG_01475) CSB-YP509531DOP
CSB-EP509531DOP
CSB-BP509531DOP
CSB-MP509531DOP
CSB-EP509531DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Probable Xaa-Pro aminopeptidase P (AMPP), partial CSB-YP509532DOP
CSB-EP509532DOP
CSB-BP509532DOP
CSB-MP509532DOP
CSB-EP509532DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Probable neutral protease 2 homolog MCYG_01991 (MCYG_01991) CSB-YP509533DOP
CSB-EP509533DOP
CSB-BP509533DOP
CSB-MP509533DOP
CSB-EP509533DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Extracellular metalloprotease MCYG_03238 (MCYG_03238) CSB-YP509534DOP
CSB-EP509534DOP
CSB-BP509534DOP
CSB-MP509534DOP
CSB-EP509534DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Subtilisin-like protease 3 (SUB3) CSB-YP509535DOP
CSB-EP509535DOP
CSB-BP509535DOP
CSB-MP509535DOP
CSB-EP509535DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Putative metallocarboxypeptidase ECM14 (ECM14), partial CSB-YP509536DOP
CSB-EP509536DOP
CSB-BP509536DOP
CSB-MP509536DOP
CSB-EP509536DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae rRNA biogenesis protein RRP36 (RRP36) CSB-YP509537DOP
CSB-EP509537DOP
CSB-BP509537DOP
CSB-MP509537DOP
CSB-EP509537DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Probable D-xylulose kinase A (xkiA), partial CSB-YP509538DOP
CSB-EP509538DOP
CSB-BP509538DOP
CSB-MP509538DOP
CSB-EP509538DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Metallocarboxypeptidase A (MCPA) CSB-YP509539DOP
CSB-EP509539DOP
CSB-BP509539DOP
CSB-MP509539DOP
CSB-EP509539DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Probable neutral protease 2 homolog MCYG_07155 (MCYG_07155) CSB-YP509540DOP
CSB-EP509540DOP
CSB-BP509540DOP
CSB-MP509540DOP
CSB-EP509540DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Subtilisin-like protease 11 (SUB11) CSB-YP509541DOP
CSB-EP509541DOP
CSB-BP509541DOP
CSB-MP509541DOP
CSB-EP509541DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Probable leucine aminopeptidase MCYG_08380 (MCYG_08380) CSB-YP509542DOP
CSB-EP509542DOP
CSB-BP509542DOP
CSB-MP509542DOP
CSB-EP509542DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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