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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Sulfolobus islandicus Probable glycine dehydrogenase [decarboxylating] subunit 2 (gcvPB), partial CSB-YP503203FPG
CSB-EP503203FPG
CSB-BP503203FPG
CSB-MP503203FPG
CSB-EP503203FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP503204FPG
CSB-EP503204FPG
CSB-BP503204FPG
CSB-MP503204FPG
CSB-EP503204FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Glutamyl-tRNA (Gln) amidotransferase subunit E, partial CSB-YP503205FPG
CSB-EP503205FPG
CSB-BP503205FPG
CSB-MP503205FPG
CSB-EP503205FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Glutamyl-tRNA (Gln) amidotransferase subunit D CSB-YP503206FPG
CSB-EP503206FPG
CSB-BP503206FPG
CSB-MP503206FPG
CSB-EP503206FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase (flpA) CSB-YP503207FPG
CSB-EP503207FPG
CSB-BP503207FPG
CSB-MP503207FPG
CSB-EP503207FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Putative HTH-type transcriptional regulatory protein YN1551_1579 (YN1551_1579) CSB-YP503208FPG
CSB-EP503208FPG
CSB-BP503208FPG
CSB-MP503208FPG
CSB-EP503208FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus tRNA (guanine (26)-N (2))-dimethyltransferase CSB-YP503209FPG
CSB-EP503209FPG
CSB-BP503209FPG
CSB-MP503209FPG
CSB-EP503209FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP503210FPG
CSB-EP503210FPG
CSB-BP503210FPG
CSB-MP503210FPG
CSB-EP503210FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Probable deoxyhypusine synthase (dys) CSB-YP503211FPG
CSB-EP503211FPG
CSB-BP503211FPG
CSB-MP503211FPG
CSB-EP503211FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Type 2 DNA topoisomerase 6 subunit B (top6B), partial CSB-YP503212FPG
CSB-EP503212FPG
CSB-BP503212FPG
CSB-MP503212FPG
CSB-EP503212FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Translation initiation factor 5A (eIF5A) CSB-YP503213FPG
CSB-EP503213FPG
CSB-BP503213FPG
CSB-MP503213FPG
CSB-EP503213FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Signal recognition 54 kDa protein (srp54) CSB-YP503214FPG
CSB-EP503214FPG
CSB-BP503214FPG
CSB-MP503214FPG
CSB-EP503214FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Chromatin protein Cren7 (creN7) CSB-YP503215FPG
CSB-EP503215FPG
CSB-BP503215FPG
CSB-MP503215FPG
CSB-EP503215FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Uridylate kinase (pyrH) CSB-YP503216FPG
CSB-EP503216FPG
CSB-BP503216FPG
CSB-MP503216FPG
CSB-EP503216FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Putative homocitrate synthase (YN1551_1618) CSB-YP503217FPG
CSB-EP503217FPG
CSB-BP503217FPG
CSB-MP503217FPG
CSB-EP503217FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus CCA-adding enzyme (cca) CSB-YP503218FPG
CSB-EP503218FPG
CSB-BP503218FPG
CSB-MP503218FPG
CSB-EP503218FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus UPF0200 protein YN1551_1675 (YN1551_1675) CSB-YP503219FPG
CSB-EP503219FPG
CSB-BP503219FPG
CSB-MP503219FPG
CSB-EP503219FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus UPF0201 protein YN1551_1676 (YN1551_1676) CSB-YP503220FPG
CSB-EP503220FPG
CSB-BP503220FPG
CSB-MP503220FPG
CSB-EP503220FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Ribonuclease Z (rnz) CSB-YP503221FPG
CSB-EP503221FPG
CSB-BP503221FPG
CSB-MP503221FPG
CSB-EP503221FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus islandicus Probable DNA primase small subunit (priA) CSB-YP503222FPG
CSB-EP503222FPG
CSB-BP503222FPG
CSB-MP503222FPG
CSB-EP503222FPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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