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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus anthracis Tryptophan synthase alpha chain (trpA) CSB-YP501607BQG
CSB-EP501607BQG
CSB-BP501607BQG
CSB-MP501607BQG
CSB-EP501607BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Tryptophan synthase beta chain (trpB) CSB-YP501608BQG
CSB-EP501608BQG
CSB-BP501608BQG
CSB-MP501608BQG
CSB-EP501608BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP501609BQG
CSB-EP501609BQG
CSB-BP501609BQG
CSB-MP501609BQG
CSB-EP501609BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Indole-3-glycerol phosphate synthase (trpC) CSB-YP501610BQG
CSB-EP501610BQG
CSB-BP501610BQG
CSB-MP501610BQG
CSB-EP501610BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Anthranilate phosphoribosyltransferase (trpD) CSB-YP501611BQG
CSB-EP501611BQG
CSB-BP501611BQG
CSB-MP501611BQG
CSB-EP501611BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Leucine--tRNA ligase (leuS), partial CSB-YP501612BQG
CSB-EP501612BQG
CSB-BP501612BQG
CSB-MP501612BQG
CSB-EP501612BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Probable molybdopterin-guanine dinucleotide biosynthesis protein A (mobA) CSB-YP501613BQG
CSB-EP501613BQG
CSB-BP501613BQG
CSB-MP501613BQG
CSB-EP501613BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis S-adenosylmethionine synthase (metK) CSB-YP501614BQG
CSB-EP501614BQG
CSB-BP501614BQG
CSB-MP501614BQG
CSB-EP501614BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis S-ribosylhomocysteine lyase (luxS) CSB-YP501615BQG
CSB-EP501615BQG
CSB-BP501615BQG
CSB-MP501615BQG
CSB-EP501615BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Putative membrane protein insertion efficiency factor (BAMEG_5083) CSB-YP501616BQG
CSB-EP501616BQG
CSB-BP501616BQG
CSB-MP501616BQG
CSB-EP501616BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 2-succinylbenzoate--CoA ligase (menE) CSB-YP501617BQG
CSB-EP501617BQG
CSB-BP501617BQG
CSB-MP501617BQG
CSB-EP501617BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0303 protein BAMEG_1180 (BAMEG_1180) CSB-YP501618BQG
CSB-EP501618BQG
CSB-BP501618BQG
CSB-MP501618BQG
CSB-EP501618BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0316 protein BAMEG_1207 (BAMEG_1207), partial CSB-YP501619BQG1
CSB-EP501619BQG1
CSB-BP501619BQG1
CSB-MP501619BQG1
CSB-EP501619BQG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0477 protein BAMEG_3349 (BAMEG_3349) CSB-YP501620BQG
CSB-EP501620BQG
CSB-BP501620BQG
CSB-MP501620BQG
CSB-EP501620BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Bis (5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] CSB-YP501621BQG
CSB-EP501621BQG
CSB-BP501621BQG
CSB-MP501621BQG
CSB-EP501621BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0738 protein BAMEG_3378 (BAMEG_3378) CSB-YP501622BQG
CSB-EP501622BQG
CSB-BP501622BQG
CSB-MP501622BQG
CSB-EP501622BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0736 protein BAMEG_3400 (BAMEG_3400) CSB-YP501623BQG
CSB-EP501623BQG
CSB-BP501623BQG
CSB-MP501623BQG
CSB-EP501623BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0234 protein BAMEG_3419 (BAMEG_3419) CSB-YP501624BQG
CSB-EP501624BQG
CSB-BP501624BQG
CSB-MP501624BQG
CSB-EP501624BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UPF0344 protein BAMEG_3427 (BAMEG_3427), partial CSB-YP501625BQG1
CSB-EP501625BQG1
CSB-BP501625BQG1
CSB-MP501625BQG1
CSB-EP501625BQG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP501626BQG
CSB-EP501626BQG
CSB-BP501626BQG
CSB-MP501626BQG
CSB-EP501626BQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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