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重組蛋白

產品名稱 貨號 規格
Recombinant Brucella melitensis biotype 2 DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP499706BWT
CSB-EP499706BWT
CSB-BP499706BWT
CSB-MP499706BWT
CSB-EP499706BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Putative cobalt-precorrin-6A synthase [deacetylating] (cbiD) CSB-YP499707BWT
CSB-EP499707BWT
CSB-BP499707BWT
CSB-MP499707BWT
CSB-EP499707BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 60 kDa chaperonin (groL), partial CSB-YP499708BWT
CSB-EP499708BWT
CSB-BP499708BWT
CSB-MP499708BWT
CSB-EP499708BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Cell division topological specificity factor (minE) CSB-YP499709BWT
CSB-EP499709BWT
CSB-BP499709BWT
CSB-MP499709BWT
CSB-EP499709BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Octanoyltransferase (lipB) CSB-YP499710BWT
CSB-EP499710BWT
CSB-BP499710BWT
CSB-MP499710BWT
CSB-EP499710BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 50S ribosomal protein L33 (rpmG) CSB-YP499711BWT
CSB-EP499711BWT
CSB-BP499711BWT
CSB-MP499711BWT
CSB-EP499711BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Bifunctional protein FolD (folD) CSB-YP499712BWT
CSB-EP499712BWT
CSB-BP499712BWT
CSB-MP499712BWT
CSB-EP499712BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 D-amino acid dehydrogenase small subunit (dadA) CSB-YP499713BWT
CSB-EP499713BWT
CSB-BP499713BWT
CSB-MP499713BWT
CSB-EP499713BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP499714BWT
CSB-EP499714BWT
CSB-BP499714BWT
CSB-MP499714BWT
CSB-EP499714BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccidioides brasiliensis Formation of crista junctions protein 1 (FCJ1), partial CSB-YP499715ERP1
CSB-EP499715ERP1
CSB-BP499715ERP1
CSB-MP499715ERP1
CSB-EP499715ERP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccidioides brasiliensis Nucleolar protein 9 (NOP9), partial CSB-YP499716ERP
CSB-EP499716ERP
CSB-BP499716ERP
CSB-MP499716ERP
CSB-EP499716ERP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccidioides brasiliensis Probable dipeptidyl-aminopeptidase B (DAPB), partial CSB-YP499717ERP
CSB-EP499717ERP
CSB-BP499717ERP
CSB-MP499717ERP
CSB-EP499717ERP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccidioides brasiliensis Putative metallocarboxypeptidase ECM14 (ECM14), partial CSB-YP499718ERP
CSB-EP499718ERP
CSB-BP499718ERP
CSB-MP499718ERP
CSB-EP499718ERP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccidioides brasiliensis Ubiquinone biosynthesis protein COQ4, mitochondrial (COQ4) CSB-YP499719ERP
CSB-EP499719ERP
CSB-BP499719ERP
CSB-MP499719ERP
CSB-EP499719ERP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccidioides brasiliensis Carboxypeptidase Y homolog A (CPYA) CSB-YP499720ERP
CSB-EP499720ERP
CSB-BP499720ERP
CSB-MP499720ERP
CSB-EP499720ERP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pastinaca sativa Psoralen synthase (CYP71AJ3), partial CSB-YP499721ESH
CSB-EP499721ESH
CSB-BP499721ESH
CSB-MP499721ESH
CSB-EP499721ESH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative binding protein ytlA (ytlA) CSB-YP499722BRI
CSB-EP499722BRI
CSB-BP499722BRI
CSB-MP499722BRI
CSB-EP499722BRI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative metallo-hydrolase yycJ (yycJ) CSB-YP499723BRI
CSB-EP499723BRI
CSB-BP499723BRI
CSB-MP499723BRI
CSB-EP499723BRI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0749 protein ylxW (ylxW) CSB-YP499724BRI
CSB-EP499724BRI
CSB-BP499724BRI
CSB-MP499724BRI
CSB-EP499724BRI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein yclD (yclD) CSB-YP499725BRI
CSB-EP499725BRI
CSB-BP499725BRI
CSB-MP499725BRI
CSB-EP499725BRI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業產品。重組蛋白的技術資源在重組蛋白的開發過程中,除了表達系統之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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