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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Wolbachia sp. subsp. Drosophila simulans Diaminopimelate epimerase (dapF) CSB-YP498986WBP
CSB-EP498986WBP
CSB-BP498986WBP
CSB-MP498986WBP
CSB-EP498986WBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia sp. subsp. Drosophila simulans Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP498987WBP
CSB-EP498987WBP
CSB-BP498987WBP
CSB-MP498987WBP
CSB-EP498987WBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia sp. subsp. Drosophila simulans Elongation factor P (efp) CSB-YP498988WBP
CSB-EP498988WBP
CSB-BP498988WBP
CSB-MP498988WBP
CSB-EP498988WBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia sp. subsp. Drosophila simulans Chaperone protein DnaJ (dnaJ) CSB-YP498989WBP
CSB-EP498989WBP
CSB-BP498989WBP
CSB-MP498989WBP
CSB-EP498989WBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia sp. subsp. Drosophila simulans 50S ribosomal protein L25 (rplY) CSB-YP498990WBP
CSB-EP498990WBP
CSB-BP498990WBP
CSB-MP498990WBP
CSB-EP498990WBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia sp. subsp. Drosophila simulans Putative Holliday junction resolvase (WRi_001100) CSB-YP498991WBP
CSB-EP498991WBP
CSB-BP498991WBP
CSB-MP498991WBP
CSB-EP498991WBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia sp. subsp. Drosophila simulans Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP498992WBP
CSB-EP498992WBP
CSB-BP498992WBP
CSB-MP498992WBP
CSB-EP498992WBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia sp. subsp. Drosophila simulans DNA repair protein recO (recO) CSB-YP498993WBP
CSB-EP498993WBP
CSB-BP498993WBP
CSB-MP498993WBP
CSB-EP498993WBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Wolbachia sp. subsp. Drosophila simulans UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP498994WBP
CSB-EP498994WBP
CSB-BP498994WBP
CSB-MP498994WBP
CSB-EP498994WBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 DNA ligase (ligA), partial CSB-YP498995BWT
CSB-EP498995BWT
CSB-BP498995BWT
CSB-MP498995BWT
CSB-EP498995BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP498996BWT
CSB-EP498996BWT
CSB-BP498996BWT
CSB-MP498996BWT
CSB-EP498996BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP498997BWT
CSB-EP498997BWT
CSB-BP498997BWT
CSB-MP498997BWT
CSB-EP498997BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Protein TolB (tolB) CSB-YP498998BWT
CSB-EP498998BWT
CSB-BP498998BWT
CSB-MP498998BWT
CSB-EP498998BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 50S ribosomal protein L32 (rpmF) CSB-YP498999BWT
CSB-EP498999BWT
CSB-BP498999BWT
CSB-MP498999BWT
CSB-EP498999BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Probable transaldolase (tal) CSB-YP499000BWT
CSB-EP499000BWT
CSB-BP499000BWT
CSB-MP499000BWT
CSB-EP499000BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 RNA pyrophosphohydrolase (rppH) CSB-YP499001BWT
CSB-EP499001BWT
CSB-BP499001BWT
CSB-MP499001BWT
CSB-EP499001BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 50S ribosomal protein L27 (rpmA) CSB-YP499002BWT
CSB-EP499002BWT
CSB-BP499002BWT
CSB-MP499002BWT
CSB-EP499002BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP499003BWT
CSB-EP499003BWT
CSB-BP499003BWT
CSB-MP499003BWT
CSB-EP499003BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Diaminopimelate epimerase (dapF) CSB-YP499004BWT
CSB-EP499004BWT
CSB-BP499004BWT
CSB-MP499004BWT
CSB-EP499004BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Shikimate dehydrogenase (aroE) CSB-YP499005BWT
CSB-EP499005BWT
CSB-BP499005BWT
CSB-MP499005BWT
CSB-EP499005BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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