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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cyanothece sp. Adenylosuccinate synthetase (purA) CSB-YP492025EPV
CSB-EP492025EPV
CSB-BP492025EPV
CSB-MP492025EPV
CSB-EP492025EPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Homoserine O-succinyltransferase (metA) CSB-YP492026DUL
CSB-EP492026DUL
CSB-BP492026DUL
CSB-MP492026DUL
CSB-EP492026DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum 50S ribosomal protein L21 (rplU) CSB-YP492027DUL
CSB-EP492027DUL
CSB-BP492027DUL
CSB-MP492027DUL
CSB-EP492027DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP492028DUL
CSB-EP492028DUL
CSB-BP492028DUL
CSB-MP492028DUL
CSB-EP492028DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Aspartate 1-decarboxylase (panD) CSB-YP492029DUL
CSB-EP492029DUL
CSB-BP492029DUL
CSB-MP492029DUL
CSB-EP492029DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP492030DUL
CSB-EP492030DUL
CSB-BP492030DUL
CSB-MP492030DUL
CSB-EP492030DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum D-tyrosyl-tRNA (Tyr) deacylase CSB-YP492031DUL
CSB-EP492031DUL
CSB-BP492031DUL
CSB-MP492031DUL
CSB-EP492031DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum DNA replication and repair protein recF (recF) CSB-YP492032DUL
CSB-EP492032DUL
CSB-BP492032DUL
CSB-MP492032DUL
CSB-EP492032DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum 50S ribosomal protein L32 (rpmF) CSB-YP492033DUL
CSB-EP492033DUL
CSB-BP492033DUL
CSB-MP492033DUL
CSB-EP492033DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP492034DUL
CSB-EP492034DUL
CSB-BP492034DUL
CSB-MP492034DUL
CSB-EP492034DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Adenylosuccinate synthetase (purA) CSB-YP492035DUL
CSB-EP492035DUL
CSB-BP492035DUL
CSB-MP492035DUL
CSB-EP492035DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Proline--tRNA ligase (proS) CSB-YP492036DUL
CSB-EP492036DUL
CSB-BP492036DUL
CSB-MP492036DUL
CSB-EP492036DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum 60 kDa chaperonin (groL), partial CSB-YP492037DUL
CSB-EP492037DUL
CSB-BP492037DUL
CSB-MP492037DUL
CSB-EP492037DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP492038DUL
CSB-EP492038DUL
CSB-BP492038DUL
CSB-MP492038DUL
CSB-EP492038DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Ribosome maturation factor RimM (rimM) CSB-YP492039DUL
CSB-EP492039DUL
CSB-BP492039DUL
CSB-MP492039DUL
CSB-EP492039DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum 30S ribosomal protein S10 (rpsJ) CSB-YP492040DUL
CSB-EP492040DUL
CSB-BP492040DUL
CSB-MP492040DUL
CSB-EP492040DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum 30S ribosomal protein S19 (rpsS) CSB-YP492041DUL
CSB-EP492041DUL
CSB-BP492041DUL
CSB-MP492041DUL
CSB-EP492041DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum 50S ribosomal protein L14 (rplN) CSB-YP492042DUL
CSB-EP492042DUL
CSB-BP492042DUL
CSB-MP492042DUL
CSB-EP492042DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum 50S ribosomal protein L15 (rplO) CSB-YP492043DUL
CSB-EP492043DUL
CSB-BP492043DUL
CSB-MP492043DUL
CSB-EP492043DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP492044DUL
CSB-EP492044DUL
CSB-BP492044DUL
CSB-MP492044DUL
CSB-EP492044DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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